MIRS measurement on cassava cell walls and flours. High-throughput phenotyping protocols (HTPP), WP3
The purpose of this SOP is to use MIRS to predict the cooking behaviour of cassava from different varieties by using spectra of corresponding cassava cell walls or flour. For this purpose, eighty-nine samples of cell walls from 37 different varieties (2 or 3 roots per genotype) provided by CIAT (Colombia) were analysed by using Thermo Scientific™ Nicolet™ iS50R Research FTIR Spectrometers in the 4000-400 cm-1 region. For the repeatability test, 8 spectra of the same flour sample were acquired in order to determine the optimum number of MIRS measurements (replicates) required to be representative of the sample. The RMS obtained for 5 combinations of two replicates (2-3, 1-2, 3-5, 3-7 and 6-8) are 457, 1066, 710, 1352 and 614 μabs respectively. The RMS values between 2 replicates, randomly selected, were lower than the RMS mean for all the replicates. These results indicate that two replicates of the sample result to a spectral dispersion (variability) similar to the one obtained with 8 repetitions.
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| Format: | monograph biblioteca |
| Language: | eng |
| Published: |
RTBfoods Project
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| Online Access: | http://agritrop.cirad.fr/602132/ http://agritrop.cirad.fr/602132/1/RTBfoods_SOP_MIRS%20measurement_Cassava%20cell%20walls%20%26%20flours.pdf |
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