Method for tick-borne encephalitis virus detection in raw milk products
The transmission of tick-borne encephalitis virus (TBEV) through food is rare, but can occur through the consumption of raw milk products from animals infected by tick bites. In 2020, France faced a TBEV outbreak linked to the consumption of unpasteurized goat cheese. The aim of this study was to develop and characterize a molecular method for the detection of TBEV in raw milk products based on the recent international standard PR ISO/DIS 16140-4. The TBEV recovery rates varied with the inoculation level and settings. The LOD50 and LOD95 of TBEV were 6.40 × 103 genome copies per g or per mL and 2.84 × 104 genome copies per g or per mL, respectively. The percentages of RT-qPCR inhibitions were lower than 75% and the murine norovirus (MNV-1), used as process control, was detected in all samples with a recovery rate higher than 1%, as recommended in ISO 15216. We conclude that the described method is appropriate to detect TBEV in raw milk products for routine diagnosis, and to assess potential health risks.
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Bibliographic Details
Main Authors: |
Hennechart-Collette, Catherine,
Gonzalez, Gaëlle,
Fourniol, Lisa,
Fraisse, Audrey,
Beck, Cécile,
Moutailler, Sara,
Bournez, Laure,
Dheilly, Nolwenn M.,
Lacour, Sandrine A.,
Lecollinet, Sylvie,
Martin-Latil, Sandra,
Perelle, Sylvie |
Format: | article
biblioteca
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Language: | eng |
Subjects: | Q03 - Contamination et toxicologie alimentaires,
encéphalite à tiques,
flavivirus,
lait cru,
produit alimentaire,
sécurité sanitaire des aliments,
contamination des aliments,
alimentation humaine,
aliment d'origine animale,
contrôle de qualité alimentaire,
PCR,
conformité,
réglementation,
procédure,
mode opératoire normalisé,
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Online Access: | http://agritrop.cirad.fr/601569/
http://agritrop.cirad.fr/601569/1/1-s2.0-S0740002022000272-main.pdf
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