Method for tick-borne encephalitis virus detection in raw milk products

The transmission of tick-borne encephalitis virus (TBEV) through food is rare, but can occur through the consumption of raw milk products from animals infected by tick bites. In 2020, France faced a TBEV outbreak linked to the consumption of unpasteurized goat cheese. The aim of this study was to develop and characterize a molecular method for the detection of TBEV in raw milk products based on the recent international standard PR ISO/DIS 16140-4. The TBEV recovery rates varied with the inoculation level and settings. The LOD50 and LOD95 of TBEV were 6.40 × 103 genome copies per g or per mL and 2.84 × 104 genome copies per g or per mL, respectively. The percentages of RT-qPCR inhibitions were lower than 75% and the murine norovirus (MNV-1), used as process control, was detected in all samples with a recovery rate higher than 1%, as recommended in ISO 15216. We conclude that the described method is appropriate to detect TBEV in raw milk products for routine diagnosis, and to assess potential health risks.

Bibliographic Details

Main Authors:	Hennechart-Collette, Catherine, Gonzalez, Gaëlle, Fourniol, Lisa, Fraisse, Audrey, Beck, Cécile, Moutailler, Sara, Bournez, Laure, Dheilly, Nolwenn M., Lacour, Sandrine A., Lecollinet, Sylvie, Martin-Latil, Sandra, Perelle, Sylvie
Format:	article biblioteca
Language:	eng
Subjects:	Q03 - Contamination et toxicologie alimentaires, encéphalite à tiques, flavivirus, lait cru, produit alimentaire, sécurité sanitaire des aliments, contamination des aliments, alimentation humaine, aliment d'origine animale, contrôle de qualité alimentaire, PCR, conformité, réglementation, procédure, mode opératoire normalisé, http://aims.fao.org/aos/agrovoc/c_16818, http://aims.fao.org/aos/agrovoc/c_2963, http://aims.fao.org/aos/agrovoc/c_24225, http://aims.fao.org/aos/agrovoc/c_3032, http://aims.fao.org/aos/agrovoc/c_33995, http://aims.fao.org/aos/agrovoc/c_10962, http://aims.fao.org/aos/agrovoc/c_15615, http://aims.fao.org/aos/agrovoc/c_2844, http://aims.fao.org/aos/agrovoc/c_a003edf0, http://aims.fao.org/aos/agrovoc/c_34079, http://aims.fao.org/aos/agrovoc/c_866a88e5, http://aims.fao.org/aos/agrovoc/c_6493, http://aims.fao.org/aos/agrovoc/c_6526cb9e, http://aims.fao.org/aos/agrovoc/c_b92b8d63, http://aims.fao.org/aos/agrovoc/c_3081,
Online Access:	http://agritrop.cirad.fr/601569/ http://agritrop.cirad.fr/601569/1/1-s2.0-S0740002022000272-main.pdf
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