NIN-like protein transcription factors regulate leghemoglobin genes in legume nodules

Leghemoglobins enable the endosymbiotic fixation of molecular nitrogen (N2) in legume nodules by channeling O2 for bacterial respiration while maintaining a micro-oxic environment to protect O2-sensitive nitrogenase. We found that the NIN-like protein (NLP) transcription factors NLP2 and NIN directly activate the expression of leghemoglobins through a promoter motif, resembling a “double” version of the nitrate-responsive elements (NREs) targeted by other NLPs, that has conserved orientation and position across legumes. CRISPR knockout of the NRE-like element resulted in strongly decreased expression of the associated leghemoglobin. Our findings indicate that the origins of the NLP-leghemoglobin module for O2 buffering in nodules can be traced to an ancient pairing of NLPs with nonsymbiotic hemoglobins that function in hypoxia.

Bibliographic Details

Main Authors:	Jiang, Suyu, Jardinaud, Marie-Françoise, Gao, Jinpeng, Pecrix, Yann, Wen, Jiangqi, Mysore, Kirankumar, Xu, Ping, Sanchez-Canizares, Carmen, Ruan, Yiting, Li, Qiujiu, Zhu, Meijun, Li, Fuyu, Wang, Ertao, Poole, Phillip S., Gamas, Pascal, Murray, Jeremy D.
Format:	article biblioteca
Language:	eng
Published:	AAAS Science
Subjects:	F30 - Génétique et amélioration des plantes, nodosité racinaire, transcription, facteur de transcription, léghémoglobine, légumineuse, Fixation de l'azote, Medicago truncatula, http://aims.fao.org/aos/agrovoc/c_27601, http://aims.fao.org/aos/agrovoc/c_35126, http://aims.fao.org/aos/agrovoc/c_35345, http://aims.fao.org/aos/agrovoc/c_26798, http://aims.fao.org/aos/agrovoc/c_4255, http://aims.fao.org/aos/agrovoc/c_5196, http://aims.fao.org/aos/agrovoc/c_26570,
Online Access:	http://agritrop.cirad.fr/600455/ http://agritrop.cirad.fr/600455/1/Jiang_et_al_2021_Science.pdf
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