Terroir is the main driver of the epiphytic bacterial and fungal communities of mango carposphere in Reunion Island
The diversity of both bacterial and fungal communities associated with mango surface was explored using a metabarcoding approach targeting fungal ITS2 and bacterial 16S (V3-V4) genomic regions. Fruits were collected in Reunion Island from two different orchards according to a sampling method which allowed the effect of several pre-harvest factors such as geographical location (terroir), cultivars, fruit parts, tree position in the plot, fruit position on the tree (orientation and height), as well as the harvest date to be investigated. A total of 4,266,546 fungal and 2,049,919 bacterial reads were recovered then respectively assigned to 3,153 fungal and 24,087 to bacterial amplicon sequence variants (ASVs). Alpha and beta diversity, as well as differential abundance analyses revealed variations in both bacterial and fungal communities detected on mango surfaces depended upon the studied factor. Results indicated that Burkholderiaceae (58.8%), Enterobacteriaceae (5.2%), Pseudomonadaceae (4.8%), Sphingomonadaceae (4.1%), Beijerinckiaceae (3.5%), and Microbacteriaceae (3.1%) were the dominant bacterial families across all samples. The majority of fungal sequences were assigned to Mycosphaerellaceae (34.5%), Cladosporiaceae (23.21%), Aureobasidiaceae (13.09%), Pleosporaceae (6.92%), Trichosphaeriaceae (5.17%), and Microstromatales_fam_Incertae_sedis (4.67%). For each studied location, mango fruit from each cultivar shared a core microbiome, and fruits of the same cultivar harvested in two different locations shared about 80% fungal and bacterial family taxa. The various factors tested in this study affected bacterial and fungal taxa differently, suggesting that some taxa could act as geographical (terroir) markers and in some cases as cultivar fingerprints. The ranking of the factors investigated in the present study showed that in decreasing order of importance: the plot (terroir), cultivar, fruit parts, harvest date and the position of the fruits are respectively the most impacting factors of the microbial flora, when compared to the orientation and the fruit position (height) on the tree. Overall, these findings provided insights on both bacterial and fungal diversity associated with the mango surface, their patterns from intra-fruit scale to local scale and the potential parameters shaping the mango microbiota.
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Subjects: | F40 - Écologie végétale, F60 - Physiologie et biochimie végétale, Mangifera indica, flore bactérienne, flore microbienne, variété, microbiologie, Burkholderiaceae, Enterobacteriaceae, http://aims.fao.org/aos/agrovoc/c_4575, http://aims.fao.org/aos/agrovoc/c_10916, http://aims.fao.org/aos/agrovoc/c_16367, http://aims.fao.org/aos/agrovoc/c_8157, http://aims.fao.org/aos/agrovoc/c_4800, http://aims.fao.org/aos/agrovoc/c_37846, http://aims.fao.org/aos/agrovoc/c_2580, http://aims.fao.org/aos/agrovoc/c_6543, http://aims.fao.org/aos/agrovoc/c_3081, |
Online Access: | http://agritrop.cirad.fr/597508/ http://agritrop.cirad.fr/597508/1/fmicb-11-619226.pdf |
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F40 - Écologie végétale F60 - Physiologie et biochimie végétale Mangifera indica flore bactérienne flore microbienne variété microbiologie Burkholderiaceae Enterobacteriaceae http://aims.fao.org/aos/agrovoc/c_4575 http://aims.fao.org/aos/agrovoc/c_10916 http://aims.fao.org/aos/agrovoc/c_16367 http://aims.fao.org/aos/agrovoc/c_8157 http://aims.fao.org/aos/agrovoc/c_4800 http://aims.fao.org/aos/agrovoc/c_37846 http://aims.fao.org/aos/agrovoc/c_2580 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 F40 - Écologie végétale F60 - Physiologie et biochimie végétale Mangifera indica flore bactérienne flore microbienne variété microbiologie Burkholderiaceae Enterobacteriaceae http://aims.fao.org/aos/agrovoc/c_4575 http://aims.fao.org/aos/agrovoc/c_10916 http://aims.fao.org/aos/agrovoc/c_16367 http://aims.fao.org/aos/agrovoc/c_8157 http://aims.fao.org/aos/agrovoc/c_4800 http://aims.fao.org/aos/agrovoc/c_37846 http://aims.fao.org/aos/agrovoc/c_2580 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 |
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F40 - Écologie végétale F60 - Physiologie et biochimie végétale Mangifera indica flore bactérienne flore microbienne variété microbiologie Burkholderiaceae Enterobacteriaceae http://aims.fao.org/aos/agrovoc/c_4575 http://aims.fao.org/aos/agrovoc/c_10916 http://aims.fao.org/aos/agrovoc/c_16367 http://aims.fao.org/aos/agrovoc/c_8157 http://aims.fao.org/aos/agrovoc/c_4800 http://aims.fao.org/aos/agrovoc/c_37846 http://aims.fao.org/aos/agrovoc/c_2580 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 F40 - Écologie végétale F60 - Physiologie et biochimie végétale Mangifera indica flore bactérienne flore microbienne variété microbiologie Burkholderiaceae Enterobacteriaceae http://aims.fao.org/aos/agrovoc/c_4575 http://aims.fao.org/aos/agrovoc/c_10916 http://aims.fao.org/aos/agrovoc/c_16367 http://aims.fao.org/aos/agrovoc/c_8157 http://aims.fao.org/aos/agrovoc/c_4800 http://aims.fao.org/aos/agrovoc/c_37846 http://aims.fao.org/aos/agrovoc/c_2580 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 Taibi, Ahmed Rivallan, Ronan Broussolle, Véronique Pallet, Dominique Lortal, Sylvie Meile, Jean-Christophe Constancias, Florentin Terroir is the main driver of the epiphytic bacterial and fungal communities of mango carposphere in Reunion Island |
description |
The diversity of both bacterial and fungal communities associated with mango surface was explored using a metabarcoding approach targeting fungal ITS2 and bacterial 16S (V3-V4) genomic regions. Fruits were collected in Reunion Island from two different orchards according to a sampling method which allowed the effect of several pre-harvest factors such as geographical location (terroir), cultivars, fruit parts, tree position in the plot, fruit position on the tree (orientation and height), as well as the harvest date to be investigated. A total of 4,266,546 fungal and 2,049,919 bacterial reads were recovered then respectively assigned to 3,153 fungal and 24,087 to bacterial amplicon sequence variants (ASVs). Alpha and beta diversity, as well as differential abundance analyses revealed variations in both bacterial and fungal communities detected on mango surfaces depended upon the studied factor. Results indicated that Burkholderiaceae (58.8%), Enterobacteriaceae (5.2%), Pseudomonadaceae (4.8%), Sphingomonadaceae (4.1%), Beijerinckiaceae (3.5%), and Microbacteriaceae (3.1%) were the dominant bacterial families across all samples. The majority of fungal sequences were assigned to Mycosphaerellaceae (34.5%), Cladosporiaceae (23.21%), Aureobasidiaceae (13.09%), Pleosporaceae (6.92%), Trichosphaeriaceae (5.17%), and Microstromatales_fam_Incertae_sedis (4.67%). For each studied location, mango fruit from each cultivar shared a core microbiome, and fruits of the same cultivar harvested in two different locations shared about 80% fungal and bacterial family taxa. The various factors tested in this study affected bacterial and fungal taxa differently, suggesting that some taxa could act as geographical (terroir) markers and in some cases as cultivar fingerprints. The ranking of the factors investigated in the present study showed that in decreasing order of importance: the plot (terroir), cultivar, fruit parts, harvest date and the position of the fruits are respectively the most impacting factors of the microbial flora, when compared to the orientation and the fruit position (height) on the tree. Overall, these findings provided insights on both bacterial and fungal diversity associated with the mango surface, their patterns from intra-fruit scale to local scale and the potential parameters shaping the mango microbiota. |
format |
article |
topic_facet |
F40 - Écologie végétale F60 - Physiologie et biochimie végétale Mangifera indica flore bactérienne flore microbienne variété microbiologie Burkholderiaceae Enterobacteriaceae http://aims.fao.org/aos/agrovoc/c_4575 http://aims.fao.org/aos/agrovoc/c_10916 http://aims.fao.org/aos/agrovoc/c_16367 http://aims.fao.org/aos/agrovoc/c_8157 http://aims.fao.org/aos/agrovoc/c_4800 http://aims.fao.org/aos/agrovoc/c_37846 http://aims.fao.org/aos/agrovoc/c_2580 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 |
author |
Taibi, Ahmed Rivallan, Ronan Broussolle, Véronique Pallet, Dominique Lortal, Sylvie Meile, Jean-Christophe Constancias, Florentin |
author_facet |
Taibi, Ahmed Rivallan, Ronan Broussolle, Véronique Pallet, Dominique Lortal, Sylvie Meile, Jean-Christophe Constancias, Florentin |
author_sort |
Taibi, Ahmed |
title |
Terroir is the main driver of the epiphytic bacterial and fungal communities of mango carposphere in Reunion Island |
title_short |
Terroir is the main driver of the epiphytic bacterial and fungal communities of mango carposphere in Reunion Island |
title_full |
Terroir is the main driver of the epiphytic bacterial and fungal communities of mango carposphere in Reunion Island |
title_fullStr |
Terroir is the main driver of the epiphytic bacterial and fungal communities of mango carposphere in Reunion Island |
title_full_unstemmed |
Terroir is the main driver of the epiphytic bacterial and fungal communities of mango carposphere in Reunion Island |
title_sort |
terroir is the main driver of the epiphytic bacterial and fungal communities of mango carposphere in reunion island |
url |
http://agritrop.cirad.fr/597508/ http://agritrop.cirad.fr/597508/1/fmicb-11-619226.pdf |
work_keys_str_mv |
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1792500096049348608 |
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dig-cirad-fr-5975082024-01-29T03:18:06Z http://agritrop.cirad.fr/597508/ http://agritrop.cirad.fr/597508/ Terroir is the main driver of the epiphytic bacterial and fungal communities of mango carposphere in Reunion Island. Taibi Ahmed, Rivallan Ronan, Broussolle Véronique, Pallet Dominique, Lortal Sylvie, Meile Jean-Christophe, Constancias Florentin. 2021. Frontiers in Microbiology, 11:619226, 19 p.https://doi.org/10.3389/fmicb.2020.619226 <https://doi.org/10.3389/fmicb.2020.619226> Terroir is the main driver of the epiphytic bacterial and fungal communities of mango carposphere in Reunion Island Taibi, Ahmed Rivallan, Ronan Broussolle, Véronique Pallet, Dominique Lortal, Sylvie Meile, Jean-Christophe Constancias, Florentin eng 2021 Frontiers in Microbiology F40 - Écologie végétale F60 - Physiologie et biochimie végétale Mangifera indica flore bactérienne flore microbienne variété microbiologie Burkholderiaceae Enterobacteriaceae http://aims.fao.org/aos/agrovoc/c_4575 http://aims.fao.org/aos/agrovoc/c_10916 http://aims.fao.org/aos/agrovoc/c_16367 http://aims.fao.org/aos/agrovoc/c_8157 http://aims.fao.org/aos/agrovoc/c_4800 http://aims.fao.org/aos/agrovoc/c_37846 http://aims.fao.org/aos/agrovoc/c_2580 La Réunion France http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 The diversity of both bacterial and fungal communities associated with mango surface was explored using a metabarcoding approach targeting fungal ITS2 and bacterial 16S (V3-V4) genomic regions. Fruits were collected in Reunion Island from two different orchards according to a sampling method which allowed the effect of several pre-harvest factors such as geographical location (terroir), cultivars, fruit parts, tree position in the plot, fruit position on the tree (orientation and height), as well as the harvest date to be investigated. A total of 4,266,546 fungal and 2,049,919 bacterial reads were recovered then respectively assigned to 3,153 fungal and 24,087 to bacterial amplicon sequence variants (ASVs). Alpha and beta diversity, as well as differential abundance analyses revealed variations in both bacterial and fungal communities detected on mango surfaces depended upon the studied factor. Results indicated that Burkholderiaceae (58.8%), Enterobacteriaceae (5.2%), Pseudomonadaceae (4.8%), Sphingomonadaceae (4.1%), Beijerinckiaceae (3.5%), and Microbacteriaceae (3.1%) were the dominant bacterial families across all samples. The majority of fungal sequences were assigned to Mycosphaerellaceae (34.5%), Cladosporiaceae (23.21%), Aureobasidiaceae (13.09%), Pleosporaceae (6.92%), Trichosphaeriaceae (5.17%), and Microstromatales_fam_Incertae_sedis (4.67%). For each studied location, mango fruit from each cultivar shared a core microbiome, and fruits of the same cultivar harvested in two different locations shared about 80% fungal and bacterial family taxa. The various factors tested in this study affected bacterial and fungal taxa differently, suggesting that some taxa could act as geographical (terroir) markers and in some cases as cultivar fingerprints. The ranking of the factors investigated in the present study showed that in decreasing order of importance: the plot (terroir), cultivar, fruit parts, harvest date and the position of the fruits are respectively the most impacting factors of the microbial flora, when compared to the orientation and the fruit position (height) on the tree. Overall, these findings provided insights on both bacterial and fungal diversity associated with the mango surface, their patterns from intra-fruit scale to local scale and the potential parameters shaping the mango microbiota. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/597508/1/fmicb-11-619226.pdf text cc_by info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ https://doi.org/10.3389/fmicb.2020.619226 10.3389/fmicb.2020.619226 info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2020.619226 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.3389/fmicb.2020.619226 info:eu-repo/semantics/reference/purl/https://CRAN.R-project.org/package=vegan |