Fluram induces species dependent C- and G-bands in mammalian chromosomes, revealing heterogenous distribution of chromosomal proteins
Fluram (Fluorescamine; 4-phenylspiro(furan-2(3H), l '-phthalan)-3,3 '-dione) is a fluorogenic reagent, which permits the detection of primary amines by forming highly fluorescent pyrrolinone derivatives. This reagent has been used on methanol - acetic acid fixed metaphase chromosomes of mouse and man and proved to be very effective in differentiating chromosome regions in both genomes. Mouse centromeric heterochromatin is highly reactive, showing intense fluorescence in ail centromeric regions, whereas human chromosomes show no fluorescence in such regions. In addition, a G-Jike banding pattern is also obtained in both types of chromosomes. The differential reactivity of each chromosome region showed by this method demonstrates a heterogeneous distribution of chromosome proteins, resulting in a chromosome banding pattern, which is in this case species dependent.
| Main Authors: | , , , |
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| Format: | article biblioteca |
| Language: | eng |
| Subjects: | U30 - Méthodes de recherche, 000 - Autres thèmes, mammifère, genre humain, souris, chromosome, protéine, cytogénétique, Microscopie immunofluorescente, http://aims.fao.org/aos/agrovoc/c_4560, http://aims.fao.org/aos/agrovoc/c_4586, http://aims.fao.org/aos/agrovoc/c_4795, http://aims.fao.org/aos/agrovoc/c_1598, http://aims.fao.org/aos/agrovoc/c_6259, http://aims.fao.org/aos/agrovoc/c_2089, http://aims.fao.org/aos/agrovoc/c_29617, |
| Online Access: | http://agritrop.cirad.fr/594057/ http://agritrop.cirad.fr/594057/1/Genome_1991.pdf |
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