Viral metagenomics approaches for high-resolution screening of multiplexed arthropod and plant viral communities
Viral metagenomic approaches have become essential for culture-independent and sequence-independent viral detection and characterization. This chapter describes an accurate and efficient approach to (1) concentrate viral particles from arthropods and plants, (2) remove contaminating non-encapsidated nucleic acids, (3) extract and amplify both viral DNA and RNA, and (4) analyze high-throughput sequencing (HTS) data by bioinformatics. Using this approach, up to 96 arthropod or plant samples can be multiplexed in a single HTS library.
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | book_section biblioteca |
| Language: | eng |
| Published: |
Humana Press
|
| Subjects: | H20 - Maladies des plantes, |
| Online Access: | http://agritrop.cirad.fr/587709/ http://agritrop.cirad.fr/587709/1/Franc%CC%A7ois_Chapter%20VANA_2018.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|