Molecular markers for DNA-fingerprinting in cotton
Molecular markers are long proposed as suitable for fingerprinting of crop species. With the development of the techniques the attention has shifted from protein to DNA-based markers as age-, tissue- and status-independent and, therefore, more reliable and reproducible. Several such markers (RFLP, RAPD, AFLP, SSR) were used in cotton for developing a high density genetic map for genomic studies and with the prospect for marker-assisted selection. The availability of these markers, together with the existing possibility for genetic transformation of the crop, make the development of DNA .fingerprints. for the varieties an issue of rising interest among breeders and seed companies. To the best of our knowledge no concentrated effort for developing such fingerprints has been made up to now in spite of the several reports proposing the potential usefulness of microsatellites and AFLPs in this respect. Here we present our work on comparing applicability of three types of molecular markers (microsatellites, AFLPs and SAMPLs) towards establishing a reliable, reproducible and relatively inexpensive protocol for DNA-fingerprinting for cotton. Furthermore, the intended transferability to the widest possible range of laboratories and, therefore, bringing it the closest to the breeder.s fields, was attempted through the replacement of the standard radioactive DNA labeling procedure with the less hazardous .silver staining . protocol. The outcomes and the possible extensions of the present work are discussed.
| Main Authors: | , |
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| Format: | conference_item biblioteca |
| Language: | eng |
| Published: |
ARC-IIC
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| Subjects: | F30 - Génétique et amélioration des plantes, U30 - Méthodes de recherche, |
| Online Access: | http://agritrop.cirad.fr/586397/ http://agritrop.cirad.fr/586397/1/ID586397.pdf |
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