The albicidin resistance factor AlbD is a serine endopeptidase that hydrolyzes unusual oligoaromatic-type peptides
The para-aminobenzoic acid-containing peptide albicidin is a pathogenicity factor synthesized by Xanthomonas albilineans in infections of sugar cane. Albicidin is a nanomolar inhibitor of the bacterial DNA gyrase with a strong activity against various Gram-negative bacteria. The bacterium Pantoea dispersa expresses the hydrolase AlbD, conferring natural resistance against albicidin. We show that AlbD is a novel type of endopeptidase that catalyzes the cleavage of albicidin at a peptide backbone amide bond, thus abolishing its antimicrobial activity. Additionally, we determined the minimal cleavage motif of AlbD with substrates derived by chemical synthesis. Our results clearly identify AlbD as a unique endopeptidase that is the first member of a new subfamily of peptidases. Our findings provide the molecular basis for a natural detoxification mechanism, potentially rendering a new tool in biological chemistry approaches.
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Bibliographic Details
| Main Authors: |
Vieweg, Laura,
Kretz, Julian,
Pesic, Alexander,
Kerwat, Dennis,
Grätz, Stefan,
Royer, Monique,
Cociancich, Stéphane,
Mainz, Andi,
Süssmuth, Roderich |
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| Format: | article
biblioteca
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| Language: | eng |
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| Subjects: | 000 - Autres thèmes,
H20 - Maladies des plantes,
Xanthomonas albilineans,
isomérase,
peptide,
composé aromatique,
antimicrobien,
acide benzoïque,
peptidase,
inhibiteur d'enzyme,
propriété antimicrobienne,
bactérie gram négatif,
détoxification métabolique,
activité enzymatique,
Saccharum officinarum,
biologie moléculaire,
http://aims.fao.org/aos/agrovoc/c_27422,
http://aims.fao.org/aos/agrovoc/c_3967,
http://aims.fao.org/aos/agrovoc/c_5691,
http://aims.fao.org/aos/agrovoc/c_622,
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http://aims.fao.org/aos/agrovoc/c_6727,
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| Online Access: | http://agritrop.cirad.fr/577983/
http://agritrop.cirad.fr/577983/1/2015_JACS_Vieweg_AlbD.pdf
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