Drought effects on expression of genes involved in ABA signaling pathway in roots of susceptible and tolerant clones of C. canephora
Our study aims to evaluate the expression of genes encoding elements responsible for the ABA biosynthesis, perception and transduction pathway such as CcNCED3, CcPYL7 and CcPP2C-1 in roots of clones tolerant (n° 14, 73 and 120) and susceptible (n° 22) to drought of C. canephora Conilon grown under controlled conditions(greenhouse) with or without irrigation. For each clone and water regime, total RNA was extracted from roots and the transcriptome profiles was evaluated using 454 sequencing. For these three genes, expression were also analyzed by real-time qPCR to validate the gene expression levels obtained by the RNA-seq in silico. In roots of all clones analyzed, results showed that the expression of the genes CcNCED3. CcPP2C-1 and CcPYL7 increased with water suspension particularly for the drought-tolerant clone 14.
| Main Authors: | , , , , , , , |
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| Format: | conference_item biblioteca |
| Language: | eng |
| Published: |
ASIC
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| Subjects: | F30 - Génétique et amélioration des plantes, H50 - Troubles divers des plantes, F61 - Physiologie végétale - Nutrition, |
| Online Access: | http://agritrop.cirad.fr/577350/ http://agritrop.cirad.fr/577350/1/pb236_costa.pdf |
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