High-throughput sequencing provides insights into genome variation and evolution in Salmonella Typhi
Isolates of Salmonella enterica serovar Typhi (Typhi), a human-restricted bacterial pathogen that causes typhoid, show limited genetic variation. We generated whole-genome sequences for 19 Typhi isolates using 454 (Roche) and Solexa (Illumina) technologies. Isolates, including the previously sequenced CT18 and Ty2 isolates, were selected to represent major nodes in the phylogenetic tree. Comparative analysis showed little evidence of purifying selection, antigenic variation or recombination between isolates. Rather, evolution in the Typhi population seems to be characterized by ongoing loss of gene function, consistent with a small effective population size. The lack of evidence for antigenic variation driven by immune selection is in contrast to strong adaptive selection for mutations conferring antibiotic resistance in Typhi. The observed patterns of genetic isolation and drift are consistent with the proposed key role of asymptomatic carriers of Typhi as the main reservoir of this pathogen, highlighting the need for identification and treatment of carriers.
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Format: | article biblioteca |
Language: | eng |
Subjects: | S50 - Santé humaine, Salmonella, sérotype, typhoïde, séquence nucléotidique, génome, évolution, santé publique, http://aims.fao.org/aos/agrovoc/c_6757, http://aims.fao.org/aos/agrovoc/c_32699, http://aims.fao.org/aos/agrovoc/c_29244, http://aims.fao.org/aos/agrovoc/c_27583, http://aims.fao.org/aos/agrovoc/c_3224, http://aims.fao.org/aos/agrovoc/c_2745, http://aims.fao.org/aos/agrovoc/c_6349, |
Online Access: | http://agritrop.cirad.fr/577147/ http://agritrop.cirad.fr/577147/1/ID577147.pdf |
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dig-cirad-fr-5771472024-01-28T22:49:53Z http://agritrop.cirad.fr/577147/ http://agritrop.cirad.fr/577147/ High-throughput sequencing provides insights into genome variation and evolution in Salmonella Typhi. Holt Kathryn E., Parkhill Julian, Mazzoni Camila J., Roumagnac Philippe, Weill François-Xavier, Goodhead Ian, Rance Richard, Baker Stephen, Maskell Duncan J., Wain John, Dolecek Christiane, Achtman Mark, Dougan Gordon. 2008. Nature Genetics, 40 (8) : 987-993.https://doi.org/10.1038/ng.195 <https://doi.org/10.1038/ng.195> High-throughput sequencing provides insights into genome variation and evolution in Salmonella Typhi Holt, Kathryn E. Parkhill, Julian Mazzoni, Camila J. Roumagnac, Philippe Weill, François-Xavier Goodhead, Ian Rance, Richard Baker, Stephen Maskell, Duncan J. Wain, John Dolecek, Christiane Achtman, Mark Dougan, Gordon eng 2008 Nature Genetics S50 - Santé humaine Salmonella sérotype typhoïde séquence nucléotidique génome évolution santé publique http://aims.fao.org/aos/agrovoc/c_6757 http://aims.fao.org/aos/agrovoc/c_32699 http://aims.fao.org/aos/agrovoc/c_29244 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_2745 http://aims.fao.org/aos/agrovoc/c_6349 Isolates of Salmonella enterica serovar Typhi (Typhi), a human-restricted bacterial pathogen that causes typhoid, show limited genetic variation. We generated whole-genome sequences for 19 Typhi isolates using 454 (Roche) and Solexa (Illumina) technologies. Isolates, including the previously sequenced CT18 and Ty2 isolates, were selected to represent major nodes in the phylogenetic tree. Comparative analysis showed little evidence of purifying selection, antigenic variation or recombination between isolates. Rather, evolution in the Typhi population seems to be characterized by ongoing loss of gene function, consistent with a small effective population size. The lack of evidence for antigenic variation driven by immune selection is in contrast to strong adaptive selection for mutations conferring antibiotic resistance in Typhi. The observed patterns of genetic isolation and drift are consistent with the proposed key role of asymptomatic carriers of Typhi as the main reservoir of this pathogen, highlighting the need for identification and treatment of carriers. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/577147/1/ID577147.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1038/ng.195 10.1038/ng.195 info:eu-repo/semantics/altIdentifier/doi/10.1038/ng.195 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1038/ng.195 info:eu-repo/semantics/reference/purl/http://www.nature.com/ng/journal/v40/n8/pdf/ng.195.pdf |
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S50 - Santé humaine Salmonella sérotype typhoïde séquence nucléotidique génome évolution santé publique http://aims.fao.org/aos/agrovoc/c_6757 http://aims.fao.org/aos/agrovoc/c_32699 http://aims.fao.org/aos/agrovoc/c_29244 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_2745 http://aims.fao.org/aos/agrovoc/c_6349 S50 - Santé humaine Salmonella sérotype typhoïde séquence nucléotidique génome évolution santé publique http://aims.fao.org/aos/agrovoc/c_6757 http://aims.fao.org/aos/agrovoc/c_32699 http://aims.fao.org/aos/agrovoc/c_29244 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_2745 http://aims.fao.org/aos/agrovoc/c_6349 |
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S50 - Santé humaine Salmonella sérotype typhoïde séquence nucléotidique génome évolution santé publique http://aims.fao.org/aos/agrovoc/c_6757 http://aims.fao.org/aos/agrovoc/c_32699 http://aims.fao.org/aos/agrovoc/c_29244 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_2745 http://aims.fao.org/aos/agrovoc/c_6349 S50 - Santé humaine Salmonella sérotype typhoïde séquence nucléotidique génome évolution santé publique http://aims.fao.org/aos/agrovoc/c_6757 http://aims.fao.org/aos/agrovoc/c_32699 http://aims.fao.org/aos/agrovoc/c_29244 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_2745 http://aims.fao.org/aos/agrovoc/c_6349 Holt, Kathryn E. Parkhill, Julian Mazzoni, Camila J. Roumagnac, Philippe Weill, François-Xavier Goodhead, Ian Rance, Richard Baker, Stephen Maskell, Duncan J. Wain, John Dolecek, Christiane Achtman, Mark Dougan, Gordon High-throughput sequencing provides insights into genome variation and evolution in Salmonella Typhi |
description |
Isolates of Salmonella enterica serovar Typhi (Typhi), a human-restricted bacterial pathogen that causes typhoid, show limited genetic variation. We generated whole-genome sequences for 19 Typhi isolates using 454 (Roche) and Solexa (Illumina) technologies. Isolates, including the previously sequenced CT18 and Ty2 isolates, were selected to represent major nodes in the phylogenetic tree. Comparative analysis showed little evidence of purifying selection, antigenic variation or recombination between isolates. Rather, evolution in the Typhi population seems to be characterized by ongoing loss of gene function, consistent with a small effective population size. The lack of evidence for antigenic variation driven by immune selection is in contrast to strong adaptive selection for mutations conferring antibiotic resistance in Typhi. The observed patterns of genetic isolation and drift are consistent with the proposed key role of asymptomatic carriers of Typhi as the main reservoir of this pathogen, highlighting the need for identification and treatment of carriers. |
format |
article |
topic_facet |
S50 - Santé humaine Salmonella sérotype typhoïde séquence nucléotidique génome évolution santé publique http://aims.fao.org/aos/agrovoc/c_6757 http://aims.fao.org/aos/agrovoc/c_32699 http://aims.fao.org/aos/agrovoc/c_29244 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_2745 http://aims.fao.org/aos/agrovoc/c_6349 |
author |
Holt, Kathryn E. Parkhill, Julian Mazzoni, Camila J. Roumagnac, Philippe Weill, François-Xavier Goodhead, Ian Rance, Richard Baker, Stephen Maskell, Duncan J. Wain, John Dolecek, Christiane Achtman, Mark Dougan, Gordon |
author_facet |
Holt, Kathryn E. Parkhill, Julian Mazzoni, Camila J. Roumagnac, Philippe Weill, François-Xavier Goodhead, Ian Rance, Richard Baker, Stephen Maskell, Duncan J. Wain, John Dolecek, Christiane Achtman, Mark Dougan, Gordon |
author_sort |
Holt, Kathryn E. |
title |
High-throughput sequencing provides insights into genome variation and evolution in Salmonella Typhi |
title_short |
High-throughput sequencing provides insights into genome variation and evolution in Salmonella Typhi |
title_full |
High-throughput sequencing provides insights into genome variation and evolution in Salmonella Typhi |
title_fullStr |
High-throughput sequencing provides insights into genome variation and evolution in Salmonella Typhi |
title_full_unstemmed |
High-throughput sequencing provides insights into genome variation and evolution in Salmonella Typhi |
title_sort |
high-throughput sequencing provides insights into genome variation and evolution in salmonella typhi |
url |
http://agritrop.cirad.fr/577147/ http://agritrop.cirad.fr/577147/1/ID577147.pdf |
work_keys_str_mv |
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