Identification of quantitative trait loci linked to Ceratocystis wilt resistance in cacao
Ceratocystis wilt (CW) in cacao (Theobroma cacao L.), caused by Ceratocystis cacaofunesta, is a drastic disease that results in plant death. The pathogen was recently identified in the major cacao-producing region of Brazil-Bahia. The identification of genetic markers tightly linked to disease resistance loci is a valuable tool for the development of resistant cultivars using marker-assisted selection (MAS). Branches of 143 six-year-old individuals of an F2 Sca 6 × ICS 1 population were wounded by making a 3-mm deep cut with a sterile scalpel, and inoculated with a 20-?l drop of a spore suspension of 3 × 104 CFU/ml. The inoculation method used allowed the population to be quantitatively phenotyped. The length of the xylem discoloration followed a continuous distribution. These results imply that the resistance was quantitatively inherited. Quantitative trait loci (QTL) analysis revealed two genomic regions (in linkage groups 3 and 9) associated with CW resistance. The QTL explained individually from 6.9 to 8.6 % of the phenotypic variation. The QTL identified are crucial for identifying genes for resistance and can be applied in the genetic breeding of cacao using MAS.
| Main Authors: | , , , , , |
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| Format: | article biblioteca |
| Language: | eng |
| Subjects: | F30 - Génétique et amélioration des plantes, H20 - Maladies des plantes, Theobroma cacao, Ceratocystis, locus des caractères quantitatifs, marqueur génétique, résistance génétique, amélioration des plantes, http://aims.fao.org/aos/agrovoc/c_7713, http://aims.fao.org/aos/agrovoc/c_1463, http://aims.fao.org/aos/agrovoc/c_37974, http://aims.fao.org/aos/agrovoc/c_24030, http://aims.fao.org/aos/agrovoc/c_35130, http://aims.fao.org/aos/agrovoc/c_5956, http://aims.fao.org/aos/agrovoc/c_779, |
| Online Access: | http://agritrop.cirad.fr/566347/ http://agritrop.cirad.fr/566347/1/document_566347.pdf |
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