Autofluerescence visualization of phenolic compound uptake by microscopic and spectroscopic techniques in caco-2 cell line model
Polyphenols are the most abundant health benefits in the human diets. However, there is a little understanding on the bioavailability particularly the localization of these compounds in the human intestinal cells. In the present study, two polyphenols (quercetin, and hesperidin) chosen according to their structural characteristics, were used to evaluate the localization from their natural fluorescence properties in human intestinal Caco-2 cell lines. Through the technique of fluorescence and confocal microscopy, such compounds were found to exhibit a specific fluorescence. According to the structure of the phenolic compounds (aglycone or glycosylated), the absorptive conditions showed themselves differently. Indeed, the rate and the route of transporting through the cellular monolayer did not seem to be identical, passing time varied from 10 to 90 min depending on which type of phenolic compounds, quercetin or hesperidin. The results showed that the passage of quercetin occurred rapidly with transcellular mechanism while the passage of hesperidin was visualized with transcellular mechanism as well at the first time then paracellular mechanism after incubation time of 90 min. Furthermore, by the detection of polyphenol absorbance, the correlation was observed with cellular uptake and polyphenol visualization by confocal microscopy. (Texte intégral)
Main Authors: | , , , |
---|---|
Format: | conference_item biblioteca |
Language: | eng |
Published: |
s.n.
|
Subjects: | Q04 - Composition des produits alimentaires, L51 - Physiologie animale - Nutrition, U30 - Méthodes de recherche, |
Online Access: | http://agritrop.cirad.fr/561233/ http://agritrop.cirad.fr/561233/1/document_561233.pdf |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cirad-fr-561233 |
---|---|
record_format |
koha |
spelling |
dig-cirad-fr-5612332021-01-04T12:23:29Z http://agritrop.cirad.fr/561233/ http://agritrop.cirad.fr/561233/ Autofluerescence visualization of phenolic compound uptake by microscopic and spectroscopic techniques in caco-2 cell line model. Petchlert C., Dhuique-Mayer Claudie, Caporiccio Bertrand, Bidel Luc P.R.. 2009. In : 4th International Conference on Polyphenols and Health (ICPH2009), 7th-10th December, 2009, Yorkshire, England. s.l. : s.n., Résumé, 1 p. International Conference on Polyphenols and Health. 4, Yorkshire, Royaume-Uni, 7 Décembre 2009/10 Décembre 2009. Researchers Autofluerescence visualization of phenolic compound uptake by microscopic and spectroscopic techniques in caco-2 cell line model Petchlert, C. Dhuique-Mayer, Claudie Caporiccio, Bertrand Bidel, Luc P.R. eng 2009 s.n. 4th International Conference on Polyphenols and Health (ICPH2009), 7th-10th December, 2009, Yorkshire, England Q04 - Composition des produits alimentaires L51 - Physiologie animale - Nutrition U30 - Méthodes de recherche Polyphenols are the most abundant health benefits in the human diets. However, there is a little understanding on the bioavailability particularly the localization of these compounds in the human intestinal cells. In the present study, two polyphenols (quercetin, and hesperidin) chosen according to their structural characteristics, were used to evaluate the localization from their natural fluorescence properties in human intestinal Caco-2 cell lines. Through the technique of fluorescence and confocal microscopy, such compounds were found to exhibit a specific fluorescence. According to the structure of the phenolic compounds (aglycone or glycosylated), the absorptive conditions showed themselves differently. Indeed, the rate and the route of transporting through the cellular monolayer did not seem to be identical, passing time varied from 10 to 90 min depending on which type of phenolic compounds, quercetin or hesperidin. The results showed that the passage of quercetin occurred rapidly with transcellular mechanism while the passage of hesperidin was visualized with transcellular mechanism as well at the first time then paracellular mechanism after incubation time of 90 min. Furthermore, by the detection of polyphenol absorbance, the correlation was observed with cellular uptake and polyphenol visualization by confocal microscopy. (Texte intégral) conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/561233/1/document_561233.pdf application/pdf Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=212758 |
institution |
CIRAD FR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cirad-fr |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CIRAD Francia |
language |
eng |
topic |
Q04 - Composition des produits alimentaires L51 - Physiologie animale - Nutrition U30 - Méthodes de recherche Q04 - Composition des produits alimentaires L51 - Physiologie animale - Nutrition U30 - Méthodes de recherche |
spellingShingle |
Q04 - Composition des produits alimentaires L51 - Physiologie animale - Nutrition U30 - Méthodes de recherche Q04 - Composition des produits alimentaires L51 - Physiologie animale - Nutrition U30 - Méthodes de recherche Petchlert, C. Dhuique-Mayer, Claudie Caporiccio, Bertrand Bidel, Luc P.R. Autofluerescence visualization of phenolic compound uptake by microscopic and spectroscopic techniques in caco-2 cell line model |
description |
Polyphenols are the most abundant health benefits in the human diets. However, there is a little understanding on the bioavailability particularly the localization of these compounds in the human intestinal cells. In the present study, two polyphenols (quercetin, and hesperidin) chosen according to their structural characteristics, were used to evaluate the localization from their natural fluorescence properties in human intestinal Caco-2 cell lines. Through the technique of fluorescence and confocal microscopy, such compounds were found to exhibit a specific fluorescence. According to the structure of the phenolic compounds (aglycone or glycosylated), the absorptive conditions showed themselves differently. Indeed, the rate and the route of transporting through the cellular monolayer did not seem to be identical, passing time varied from 10 to 90 min depending on which type of phenolic compounds, quercetin or hesperidin. The results showed that the passage of quercetin occurred rapidly with transcellular mechanism while the passage of hesperidin was visualized with transcellular mechanism as well at the first time then paracellular mechanism after incubation time of 90 min. Furthermore, by the detection of polyphenol absorbance, the correlation was observed with cellular uptake and polyphenol visualization by confocal microscopy. (Texte intégral) |
format |
conference_item |
topic_facet |
Q04 - Composition des produits alimentaires L51 - Physiologie animale - Nutrition U30 - Méthodes de recherche |
author |
Petchlert, C. Dhuique-Mayer, Claudie Caporiccio, Bertrand Bidel, Luc P.R. |
author_facet |
Petchlert, C. Dhuique-Mayer, Claudie Caporiccio, Bertrand Bidel, Luc P.R. |
author_sort |
Petchlert, C. |
title |
Autofluerescence visualization of phenolic compound uptake by microscopic and spectroscopic techniques in caco-2 cell line model |
title_short |
Autofluerescence visualization of phenolic compound uptake by microscopic and spectroscopic techniques in caco-2 cell line model |
title_full |
Autofluerescence visualization of phenolic compound uptake by microscopic and spectroscopic techniques in caco-2 cell line model |
title_fullStr |
Autofluerescence visualization of phenolic compound uptake by microscopic and spectroscopic techniques in caco-2 cell line model |
title_full_unstemmed |
Autofluerescence visualization of phenolic compound uptake by microscopic and spectroscopic techniques in caco-2 cell line model |
title_sort |
autofluerescence visualization of phenolic compound uptake by microscopic and spectroscopic techniques in caco-2 cell line model |
publisher |
s.n. |
url |
http://agritrop.cirad.fr/561233/ http://agritrop.cirad.fr/561233/1/document_561233.pdf |
work_keys_str_mv |
AT petchlertc autofluerescencevisualizationofphenoliccompounduptakebymicroscopicandspectroscopictechniquesincaco2celllinemodel AT dhuiquemayerclaudie autofluerescencevisualizationofphenoliccompounduptakebymicroscopicandspectroscopictechniquesincaco2celllinemodel AT caporicciobertrand autofluerescencevisualizationofphenoliccompounduptakebymicroscopicandspectroscopictechniquesincaco2celllinemodel AT bidellucpr autofluerescencevisualizationofphenoliccompounduptakebymicroscopicandspectroscopictechniquesincaco2celllinemodel |
_version_ |
1758023003479736320 |