Application of PCR-DGGE (Polymerase chain reaction denaturing) to analyze microbial ecosystems in foods
The determination of geographical origin is a requirement of traceability system of import and export of food products. To this end, molecular techniques using 16S, 26S rDNA profiles generated by PCR-DGGE were used to detect the variation of the microbial community (bacteria, yeasts) structures of Pangasius fish from Viet Nam harvested in different aquaculture farms and during different seasons and two fruits from Physalis. If Physalis, the ecology of the yeast was also studied in the same purpose. Bacterial band profiles and mandarin fish, yeast and the Gaza Physalis specific profiles for each location and can be used as a bar code to certify the origin of fish and fruit. This method is a new tracking tool, which provides organic food with a unique bar code and an audit trail of food to their original location.
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dig-cirad-fr-5595482022-04-21T07:49:19Z http://agritrop.cirad.fr/559548/ http://agritrop.cirad.fr/559548/ Application of PCR-DGGE (Polymerase chain reaction denaturing) to analyze microbial ecosystems in foods. Brabet Catherine, Montet Didier, El Sheikha Aly, Le Nguyen Doan Duy. 2009. In : International Society for Mycotoxicology (ISM) " Worldwide mycotoxin reduction in food and feed chains", 9-11 september 2009, Vienna, Austria. s.l. : s.n., Résumé, 1 p. International Society for Mycotoxicology (ISM) Conference, Vienne, Autriche, 9 Septembre 2009/11 Septembre 2009. Researchers Application of PCR-DGGE (Polymerase chain reaction denaturing) to analyze microbial ecosystems in foods Brabet, Catherine Montet, Didier El Sheikha, Aly Le Nguyen, Doan Duy eng 2009 s.n. International Society for Mycotoxicology (ISM) " Worldwide mycotoxin reduction in food and feed chains", 9-11 september 2009, Vienna, Austria U30 - Méthodes de recherche Q04 - Composition des produits alimentaires E70 - Commerce, commercialisation et distribution The determination of geographical origin is a requirement of traceability system of import and export of food products. To this end, molecular techniques using 16S, 26S rDNA profiles generated by PCR-DGGE were used to detect the variation of the microbial community (bacteria, yeasts) structures of Pangasius fish from Viet Nam harvested in different aquaculture farms and during different seasons and two fruits from Physalis. If Physalis, the ecology of the yeast was also studied in the same purpose. Bacterial band profiles and mandarin fish, yeast and the Gaza Physalis specific profiles for each location and can be used as a bar code to certify the origin of fish and fruit. This method is a new tracking tool, which provides organic food with a unique bar code and an audit trail of food to their original location. conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/559548/1/document_559548.pdf application/pdf Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=211241 |
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U30 - Méthodes de recherche Q04 - Composition des produits alimentaires E70 - Commerce, commercialisation et distribution U30 - Méthodes de recherche Q04 - Composition des produits alimentaires E70 - Commerce, commercialisation et distribution Brabet, Catherine Montet, Didier El Sheikha, Aly Le Nguyen, Doan Duy Application of PCR-DGGE (Polymerase chain reaction denaturing) to analyze microbial ecosystems in foods |
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The determination of geographical origin is a requirement of traceability system of import and export of food products. To this end, molecular techniques using 16S, 26S rDNA profiles generated by PCR-DGGE were used to detect the variation of the microbial community (bacteria, yeasts) structures of Pangasius fish from Viet Nam harvested in different aquaculture farms and during different seasons and two fruits from Physalis. If Physalis, the ecology of the yeast was also studied in the same purpose. Bacterial band profiles and mandarin fish, yeast and the Gaza Physalis specific profiles for each location and can be used as a bar code to certify the origin of fish and fruit. This method is a new tracking tool, which provides organic food with a unique bar code and an audit trail of food to their original location. |
format |
conference_item |
topic_facet |
U30 - Méthodes de recherche Q04 - Composition des produits alimentaires E70 - Commerce, commercialisation et distribution |
author |
Brabet, Catherine Montet, Didier El Sheikha, Aly Le Nguyen, Doan Duy |
author_facet |
Brabet, Catherine Montet, Didier El Sheikha, Aly Le Nguyen, Doan Duy |
author_sort |
Brabet, Catherine |
title |
Application of PCR-DGGE (Polymerase chain reaction denaturing) to analyze microbial ecosystems in foods |
title_short |
Application of PCR-DGGE (Polymerase chain reaction denaturing) to analyze microbial ecosystems in foods |
title_full |
Application of PCR-DGGE (Polymerase chain reaction denaturing) to analyze microbial ecosystems in foods |
title_fullStr |
Application of PCR-DGGE (Polymerase chain reaction denaturing) to analyze microbial ecosystems in foods |
title_full_unstemmed |
Application of PCR-DGGE (Polymerase chain reaction denaturing) to analyze microbial ecosystems in foods |
title_sort |
application of pcr-dgge (polymerase chain reaction denaturing) to analyze microbial ecosystems in foods |
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s.n. |
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http://agritrop.cirad.fr/559548/ http://agritrop.cirad.fr/559548/1/document_559548.pdf |
work_keys_str_mv |
AT brabetcatherine applicationofpcrdggepolymerasechainreactiondenaturingtoanalyzemicrobialecosystemsinfoods AT montetdidier applicationofpcrdggepolymerasechainreactiondenaturingtoanalyzemicrobialecosystemsinfoods AT elsheikhaaly applicationofpcrdggepolymerasechainreactiondenaturingtoanalyzemicrobialecosystemsinfoods AT lenguyendoanduy applicationofpcrdggepolymerasechainreactiondenaturingtoanalyzemicrobialecosystemsinfoods |
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1758022858049585152 |