Study of the microbial diversity of Oreochromis niloticus of three lakes of Cameroon by PCR-DGGE: Application to the determination of the geographical origin
The new European regulation 178/2002 imposes the determination of the geographical origin in the traceability process of the foodstuffs at the moment of commercial transactions. In practice, it is difficult to determine with accuracy the geographical origin of foodstuffs. For this purpose, the total analysis of the bacterial communities in samples is used. In the present study the molecular technique using 16S rDNA profiles generated by PCR-DGGE was used in order to detect the variation in bacterial community structures of Tilapia fish from three different lakes of the north of Cameroon and the effect of the season and fish species on these bacteria profiles. The V3 region of bacterial rDNA from fish was amplified by PCR and was analyzed by DGGE. When the 16S rDNA profiles were analysed by multivariate analysis, distinct microbial communities were detected. The bands profiles obtained from different lakes were different and specific for each location and could be used as a bar code to certify the origin of the fish. The fish specie did not have an influence on microbial profiles of fish contrarily to the season.
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Subjects: | Q04 - Composition des produits alimentaires, U30 - Méthodes de recherche, E70 - Commerce, commercialisation et distribution, M01 - Pêche et aquaculture - Considérations générales, Tilapia, Oreochromis niloticus, flore microbienne, provenance, PCR, électrophorèse sur gel, http://aims.fao.org/aos/agrovoc/c_32720, http://aims.fao.org/aos/agrovoc/c_34687, http://aims.fao.org/aos/agrovoc/c_16367, http://aims.fao.org/aos/agrovoc/c_16022, http://aims.fao.org/aos/agrovoc/c_34079, http://aims.fao.org/aos/agrovoc/c_34949, http://aims.fao.org/aos/agrovoc/c_1229, |
Online Access: | http://agritrop.cirad.fr/552898/ http://agritrop.cirad.fr/552898/1/document_552898.pdf |
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dig-cirad-fr-5528982024-01-28T17:56:31Z http://agritrop.cirad.fr/552898/ http://agritrop.cirad.fr/552898/ Study of the microbial diversity of Oreochromis niloticus of three lakes of Cameroon by PCR-DGGE: Application to the determination of the geographical origin. Tatsadjieu Ngoune Léopold, Maïworé Justine, Hadjia M.B., Loiseau Gérard, Montet Didier, Mbofung C.M.F.. 2010. Food Control, 21 (5) : 673-678.https://doi.org/10.1016/j.foodcont.2009.10.006 <https://doi.org/10.1016/j.foodcont.2009.10.006> Study of the microbial diversity of Oreochromis niloticus of three lakes of Cameroon by PCR-DGGE: Application to the determination of the geographical origin Tatsadjieu, Ngoune Léopold Maïworé, Justine Hadjia, M.B. Loiseau, Gérard Montet, Didier Mbofung, C.M.F. eng 2010 Food Control Q04 - Composition des produits alimentaires U30 - Méthodes de recherche E70 - Commerce, commercialisation et distribution M01 - Pêche et aquaculture - Considérations générales Tilapia Oreochromis niloticus flore microbienne provenance PCR électrophorèse sur gel http://aims.fao.org/aos/agrovoc/c_32720 http://aims.fao.org/aos/agrovoc/c_34687 http://aims.fao.org/aos/agrovoc/c_16367 http://aims.fao.org/aos/agrovoc/c_16022 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_34949 Cameroun http://aims.fao.org/aos/agrovoc/c_1229 The new European regulation 178/2002 imposes the determination of the geographical origin in the traceability process of the foodstuffs at the moment of commercial transactions. In practice, it is difficult to determine with accuracy the geographical origin of foodstuffs. For this purpose, the total analysis of the bacterial communities in samples is used. In the present study the molecular technique using 16S rDNA profiles generated by PCR-DGGE was used in order to detect the variation in bacterial community structures of Tilapia fish from three different lakes of the north of Cameroon and the effect of the season and fish species on these bacteria profiles. The V3 region of bacterial rDNA from fish was amplified by PCR and was analyzed by DGGE. When the 16S rDNA profiles were analysed by multivariate analysis, distinct microbial communities were detected. The bands profiles obtained from different lakes were different and specific for each location and could be used as a bar code to certify the origin of the fish. The fish specie did not have an influence on microbial profiles of fish contrarily to the season. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/552898/1/document_552898.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.foodcont.2009.10.006 10.1016/j.foodcont.2009.10.006 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodcont.2009.10.006 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.foodcont.2009.10.006 |
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Q04 - Composition des produits alimentaires U30 - Méthodes de recherche E70 - Commerce, commercialisation et distribution M01 - Pêche et aquaculture - Considérations générales Tilapia Oreochromis niloticus flore microbienne provenance PCR électrophorèse sur gel http://aims.fao.org/aos/agrovoc/c_32720 http://aims.fao.org/aos/agrovoc/c_34687 http://aims.fao.org/aos/agrovoc/c_16367 http://aims.fao.org/aos/agrovoc/c_16022 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_34949 http://aims.fao.org/aos/agrovoc/c_1229 Q04 - Composition des produits alimentaires U30 - Méthodes de recherche E70 - Commerce, commercialisation et distribution M01 - Pêche et aquaculture - Considérations générales Tilapia Oreochromis niloticus flore microbienne provenance PCR électrophorèse sur gel http://aims.fao.org/aos/agrovoc/c_32720 http://aims.fao.org/aos/agrovoc/c_34687 http://aims.fao.org/aos/agrovoc/c_16367 http://aims.fao.org/aos/agrovoc/c_16022 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_34949 http://aims.fao.org/aos/agrovoc/c_1229 |
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Q04 - Composition des produits alimentaires U30 - Méthodes de recherche E70 - Commerce, commercialisation et distribution M01 - Pêche et aquaculture - Considérations générales Tilapia Oreochromis niloticus flore microbienne provenance PCR électrophorèse sur gel http://aims.fao.org/aos/agrovoc/c_32720 http://aims.fao.org/aos/agrovoc/c_34687 http://aims.fao.org/aos/agrovoc/c_16367 http://aims.fao.org/aos/agrovoc/c_16022 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_34949 http://aims.fao.org/aos/agrovoc/c_1229 Q04 - Composition des produits alimentaires U30 - Méthodes de recherche E70 - Commerce, commercialisation et distribution M01 - Pêche et aquaculture - Considérations générales Tilapia Oreochromis niloticus flore microbienne provenance PCR électrophorèse sur gel http://aims.fao.org/aos/agrovoc/c_32720 http://aims.fao.org/aos/agrovoc/c_34687 http://aims.fao.org/aos/agrovoc/c_16367 http://aims.fao.org/aos/agrovoc/c_16022 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_34949 http://aims.fao.org/aos/agrovoc/c_1229 Tatsadjieu, Ngoune Léopold Maïworé, Justine Hadjia, M.B. Loiseau, Gérard Montet, Didier Mbofung, C.M.F. Study of the microbial diversity of Oreochromis niloticus of three lakes of Cameroon by PCR-DGGE: Application to the determination of the geographical origin |
description |
The new European regulation 178/2002 imposes the determination of the geographical origin in the traceability process of the foodstuffs at the moment of commercial transactions. In practice, it is difficult to determine with accuracy the geographical origin of foodstuffs. For this purpose, the total analysis of the bacterial communities in samples is used. In the present study the molecular technique using 16S rDNA profiles generated by PCR-DGGE was used in order to detect the variation in bacterial community structures of Tilapia fish from three different lakes of the north of Cameroon and the effect of the season and fish species on these bacteria profiles. The V3 region of bacterial rDNA from fish was amplified by PCR and was analyzed by DGGE. When the 16S rDNA profiles were analysed by multivariate analysis, distinct microbial communities were detected. The bands profiles obtained from different lakes were different and specific for each location and could be used as a bar code to certify the origin of the fish. The fish specie did not have an influence on microbial profiles of fish contrarily to the season. |
format |
article |
topic_facet |
Q04 - Composition des produits alimentaires U30 - Méthodes de recherche E70 - Commerce, commercialisation et distribution M01 - Pêche et aquaculture - Considérations générales Tilapia Oreochromis niloticus flore microbienne provenance PCR électrophorèse sur gel http://aims.fao.org/aos/agrovoc/c_32720 http://aims.fao.org/aos/agrovoc/c_34687 http://aims.fao.org/aos/agrovoc/c_16367 http://aims.fao.org/aos/agrovoc/c_16022 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_34949 http://aims.fao.org/aos/agrovoc/c_1229 |
author |
Tatsadjieu, Ngoune Léopold Maïworé, Justine Hadjia, M.B. Loiseau, Gérard Montet, Didier Mbofung, C.M.F. |
author_facet |
Tatsadjieu, Ngoune Léopold Maïworé, Justine Hadjia, M.B. Loiseau, Gérard Montet, Didier Mbofung, C.M.F. |
author_sort |
Tatsadjieu, Ngoune Léopold |
title |
Study of the microbial diversity of Oreochromis niloticus of three lakes of Cameroon by PCR-DGGE: Application to the determination of the geographical origin |
title_short |
Study of the microbial diversity of Oreochromis niloticus of three lakes of Cameroon by PCR-DGGE: Application to the determination of the geographical origin |
title_full |
Study of the microbial diversity of Oreochromis niloticus of three lakes of Cameroon by PCR-DGGE: Application to the determination of the geographical origin |
title_fullStr |
Study of the microbial diversity of Oreochromis niloticus of three lakes of Cameroon by PCR-DGGE: Application to the determination of the geographical origin |
title_full_unstemmed |
Study of the microbial diversity of Oreochromis niloticus of three lakes of Cameroon by PCR-DGGE: Application to the determination of the geographical origin |
title_sort |
study of the microbial diversity of oreochromis niloticus of three lakes of cameroon by pcr-dgge: application to the determination of the geographical origin |
url |
http://agritrop.cirad.fr/552898/ http://agritrop.cirad.fr/552898/1/document_552898.pdf |
work_keys_str_mv |
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