Sequence analysis of the large (L) polymerase gene and trailer of the peste des petits ruminants virus vaccine strain Nigeria 75/1: Expression and use of the L protein in reverse genetics
The large (L) polymerase gene and the 5_-terminal UTR of the genome of peste des petits ruminants virus (PPRV), vaccine strain Nigeria 75/1, were cloned and sequenced. The L protein was also expressed in eukaryotic cells and its polymerase activity was quantitatively measured in a PPR reverse genetics assay using a reporter minigenome.Comparative sequence analysis of this functional L gene with corresponding genes of other morbilliviruses showed a degree of conservation exceeding 70%. The multiple sequence alignment and the phylogenetic study of L gene discriminated the morbilliviruses in 6 clusters, which are more closely related to Tupaia and Henipaviruses than to other paramyxoviruses. Important protein domains and functional motifs of the L polymerase of the PPRV Nigeria 75/1 vaccine were also identified by using different bioinformatics tools.
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Subjects: | L73 - Maladies des animaux, ruminant, morbillivirus, Paramyxoviridae, virus peste petits ruminants, vaccin, peste des petits ruminants, enzyme, petits ruminants, http://aims.fao.org/aos/agrovoc/c_6695, http://aims.fao.org/aos/agrovoc/c_4937, http://aims.fao.org/aos/agrovoc/c_5567, http://aims.fao.org/aos/agrovoc/c_16581, http://aims.fao.org/aos/agrovoc/c_8130, http://aims.fao.org/aos/agrovoc/c_16789, http://aims.fao.org/aos/agrovoc/c_2603, http://aims.fao.org/aos/agrovoc/c_32579, http://aims.fao.org/aos/agrovoc/c_5182, |
Online Access: | http://agritrop.cirad.fr/551325/ http://agritrop.cirad.fr/551325/1/document_551325.pdf |
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dig-cirad-fr-5513252024-01-28T17:35:42Z http://agritrop.cirad.fr/551325/ http://agritrop.cirad.fr/551325/ Sequence analysis of the large (L) polymerase gene and trailer of the peste des petits ruminants virus vaccine strain Nigeria 75/1: Expression and use of the L protein in reverse genetics. Minet Cécile, Yami Martha, Egziabher Berhe G., Gil Patricia, Tangy F., Bremont M., Libeau Geneviève, Diallo Adama, Albina Emmanuel. 2009. Virus Research, 145 (1) : 9-17.https://doi.org/10.1016/j.virusres.2009.06.002 <https://doi.org/10.1016/j.virusres.2009.06.002> Sequence analysis of the large (L) polymerase gene and trailer of the peste des petits ruminants virus vaccine strain Nigeria 75/1: Expression and use of the L protein in reverse genetics Minet, Cécile Yami, Martha Egziabher, Berhe G. Gil, Patricia Tangy, F. Bremont, M. Libeau, Geneviève Diallo, Adama Albina, Emmanuel eng 2009 Virus Research L73 - Maladies des animaux ruminant morbillivirus Paramyxoviridae virus peste petits ruminants vaccin peste des petits ruminants enzyme petits ruminants http://aims.fao.org/aos/agrovoc/c_6695 http://aims.fao.org/aos/agrovoc/c_4937 http://aims.fao.org/aos/agrovoc/c_5567 http://aims.fao.org/aos/agrovoc/c_16581 http://aims.fao.org/aos/agrovoc/c_8130 http://aims.fao.org/aos/agrovoc/c_16789 http://aims.fao.org/aos/agrovoc/c_2603 http://aims.fao.org/aos/agrovoc/c_32579 Nigéria http://aims.fao.org/aos/agrovoc/c_5182 The large (L) polymerase gene and the 5_-terminal UTR of the genome of peste des petits ruminants virus (PPRV), vaccine strain Nigeria 75/1, were cloned and sequenced. The L protein was also expressed in eukaryotic cells and its polymerase activity was quantitatively measured in a PPR reverse genetics assay using a reporter minigenome.Comparative sequence analysis of this functional L gene with corresponding genes of other morbilliviruses showed a degree of conservation exceeding 70%. The multiple sequence alignment and the phylogenetic study of L gene discriminated the morbilliviruses in 6 clusters, which are more closely related to Tupaia and Henipaviruses than to other paramyxoviruses. Important protein domains and functional motifs of the L polymerase of the PPRV Nigeria 75/1 vaccine were also identified by using different bioinformatics tools. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/551325/1/document_551325.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.virusres.2009.06.002 10.1016/j.virusres.2009.06.002 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=201419 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.virusres.2009.06.002 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.virusres.2009.06.002 |
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L73 - Maladies des animaux ruminant morbillivirus Paramyxoviridae virus peste petits ruminants vaccin peste des petits ruminants enzyme petits ruminants http://aims.fao.org/aos/agrovoc/c_6695 http://aims.fao.org/aos/agrovoc/c_4937 http://aims.fao.org/aos/agrovoc/c_5567 http://aims.fao.org/aos/agrovoc/c_16581 http://aims.fao.org/aos/agrovoc/c_8130 http://aims.fao.org/aos/agrovoc/c_16789 http://aims.fao.org/aos/agrovoc/c_2603 http://aims.fao.org/aos/agrovoc/c_32579 http://aims.fao.org/aos/agrovoc/c_5182 L73 - Maladies des animaux ruminant morbillivirus Paramyxoviridae virus peste petits ruminants vaccin peste des petits ruminants enzyme petits ruminants http://aims.fao.org/aos/agrovoc/c_6695 http://aims.fao.org/aos/agrovoc/c_4937 http://aims.fao.org/aos/agrovoc/c_5567 http://aims.fao.org/aos/agrovoc/c_16581 http://aims.fao.org/aos/agrovoc/c_8130 http://aims.fao.org/aos/agrovoc/c_16789 http://aims.fao.org/aos/agrovoc/c_2603 http://aims.fao.org/aos/agrovoc/c_32579 http://aims.fao.org/aos/agrovoc/c_5182 |
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L73 - Maladies des animaux ruminant morbillivirus Paramyxoviridae virus peste petits ruminants vaccin peste des petits ruminants enzyme petits ruminants http://aims.fao.org/aos/agrovoc/c_6695 http://aims.fao.org/aos/agrovoc/c_4937 http://aims.fao.org/aos/agrovoc/c_5567 http://aims.fao.org/aos/agrovoc/c_16581 http://aims.fao.org/aos/agrovoc/c_8130 http://aims.fao.org/aos/agrovoc/c_16789 http://aims.fao.org/aos/agrovoc/c_2603 http://aims.fao.org/aos/agrovoc/c_32579 http://aims.fao.org/aos/agrovoc/c_5182 L73 - Maladies des animaux ruminant morbillivirus Paramyxoviridae virus peste petits ruminants vaccin peste des petits ruminants enzyme petits ruminants http://aims.fao.org/aos/agrovoc/c_6695 http://aims.fao.org/aos/agrovoc/c_4937 http://aims.fao.org/aos/agrovoc/c_5567 http://aims.fao.org/aos/agrovoc/c_16581 http://aims.fao.org/aos/agrovoc/c_8130 http://aims.fao.org/aos/agrovoc/c_16789 http://aims.fao.org/aos/agrovoc/c_2603 http://aims.fao.org/aos/agrovoc/c_32579 http://aims.fao.org/aos/agrovoc/c_5182 Minet, Cécile Yami, Martha Egziabher, Berhe G. Gil, Patricia Tangy, F. Bremont, M. Libeau, Geneviève Diallo, Adama Albina, Emmanuel Sequence analysis of the large (L) polymerase gene and trailer of the peste des petits ruminants virus vaccine strain Nigeria 75/1: Expression and use of the L protein in reverse genetics |
description |
The large (L) polymerase gene and the 5_-terminal UTR of the genome of peste des petits ruminants virus (PPRV), vaccine strain Nigeria 75/1, were cloned and sequenced. The L protein was also expressed in eukaryotic cells and its polymerase activity was quantitatively measured in a PPR reverse genetics assay using a reporter minigenome.Comparative sequence analysis of this functional L gene with corresponding genes of other morbilliviruses showed a degree of conservation exceeding 70%. The multiple sequence alignment and the phylogenetic study of L gene discriminated the morbilliviruses in 6 clusters, which are more closely related to Tupaia and Henipaviruses than to other paramyxoviruses. Important protein domains and functional motifs of the L polymerase of the PPRV Nigeria 75/1 vaccine were also identified by using different bioinformatics tools. |
format |
article |
topic_facet |
L73 - Maladies des animaux ruminant morbillivirus Paramyxoviridae virus peste petits ruminants vaccin peste des petits ruminants enzyme petits ruminants http://aims.fao.org/aos/agrovoc/c_6695 http://aims.fao.org/aos/agrovoc/c_4937 http://aims.fao.org/aos/agrovoc/c_5567 http://aims.fao.org/aos/agrovoc/c_16581 http://aims.fao.org/aos/agrovoc/c_8130 http://aims.fao.org/aos/agrovoc/c_16789 http://aims.fao.org/aos/agrovoc/c_2603 http://aims.fao.org/aos/agrovoc/c_32579 http://aims.fao.org/aos/agrovoc/c_5182 |
author |
Minet, Cécile Yami, Martha Egziabher, Berhe G. Gil, Patricia Tangy, F. Bremont, M. Libeau, Geneviève Diallo, Adama Albina, Emmanuel |
author_facet |
Minet, Cécile Yami, Martha Egziabher, Berhe G. Gil, Patricia Tangy, F. Bremont, M. Libeau, Geneviève Diallo, Adama Albina, Emmanuel |
author_sort |
Minet, Cécile |
title |
Sequence analysis of the large (L) polymerase gene and trailer of the peste des petits ruminants virus vaccine strain Nigeria 75/1: Expression and use of the L protein in reverse genetics |
title_short |
Sequence analysis of the large (L) polymerase gene and trailer of the peste des petits ruminants virus vaccine strain Nigeria 75/1: Expression and use of the L protein in reverse genetics |
title_full |
Sequence analysis of the large (L) polymerase gene and trailer of the peste des petits ruminants virus vaccine strain Nigeria 75/1: Expression and use of the L protein in reverse genetics |
title_fullStr |
Sequence analysis of the large (L) polymerase gene and trailer of the peste des petits ruminants virus vaccine strain Nigeria 75/1: Expression and use of the L protein in reverse genetics |
title_full_unstemmed |
Sequence analysis of the large (L) polymerase gene and trailer of the peste des petits ruminants virus vaccine strain Nigeria 75/1: Expression and use of the L protein in reverse genetics |
title_sort |
sequence analysis of the large (l) polymerase gene and trailer of the peste des petits ruminants virus vaccine strain nigeria 75/1: expression and use of the l protein in reverse genetics |
url |
http://agritrop.cirad.fr/551325/ http://agritrop.cirad.fr/551325/1/document_551325.pdf |
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