Sequence analysis of the large (L) polymerase gene and trailer of the peste des petits ruminants virus vaccine strain Nigeria 75/1: Expression and use of the L protein in reverse genetics
The large (L) polymerase gene and the 5_-terminal UTR of the genome of peste des petits ruminants virus (PPRV), vaccine strain Nigeria 75/1, were cloned and sequenced. The L protein was also expressed in eukaryotic cells and its polymerase activity was quantitatively measured in a PPR reverse genetics assay using a reporter minigenome.Comparative sequence analysis of this functional L gene with corresponding genes of other morbilliviruses showed a degree of conservation exceeding 70%. The multiple sequence alignment and the phylogenetic study of L gene discriminated the morbilliviruses in 6 clusters, which are more closely related to Tupaia and Henipaviruses than to other paramyxoviruses. Important protein domains and functional motifs of the L polymerase of the PPRV Nigeria 75/1 vaccine were also identified by using different bioinformatics tools.
| Main Authors: | , , , , , , , , |
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| Format: | article biblioteca |
| Language: | eng |
| Subjects: | L73 - Maladies des animaux, ruminant, morbillivirus, Paramyxoviridae, virus peste petits ruminants, vaccin, peste des petits ruminants, enzyme, petits ruminants, http://aims.fao.org/aos/agrovoc/c_6695, http://aims.fao.org/aos/agrovoc/c_4937, http://aims.fao.org/aos/agrovoc/c_5567, http://aims.fao.org/aos/agrovoc/c_16581, http://aims.fao.org/aos/agrovoc/c_8130, http://aims.fao.org/aos/agrovoc/c_16789, http://aims.fao.org/aos/agrovoc/c_2603, http://aims.fao.org/aos/agrovoc/c_32579, http://aims.fao.org/aos/agrovoc/c_5182, |
| Online Access: | http://agritrop.cirad.fr/551325/ http://agritrop.cirad.fr/551325/1/document_551325.pdf |
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