Cryopreservation by encapsulation-dehydration of plumules of coconut (Cocos nucifera)

This study describes the use of an encapsulation-dehydration cryopreservation technique on coconut plumules (apical dome with three or four leaf primordia) excised from embryos. In order to establish a reliable cryopreservation process for plumules, several different key factors were tested: pretreatment duration, sugar concentration, dehydration period and freezing. In parallel, histological studies were performed to describe the structural changes of tissues and plumule cells subjected to dehydration and freezing. A good survival level of around 60% was obtained. However, after 8 months culture regrowth, this level decreased to a maximum of 20% which was achieved using sucrose treatment. In this paper we report for the first time the regeneration of leafy shoots from coconut plumules after cryopreservation.

Bibliographic Details

Main Authors:	N'Nan, Oulo, Hocher, Valérie, Verdeil, Jean-Luc, Konan Konan, Jean Louis, Ballo Koffi, C., Mondeil, Fanja, Malaurie, Bernard
Format:	article biblioteca
Language:	eng
Subjects:	F02 - Multiplication végétative des plantes, F62 - Physiologie végétale - Croissance et développement, Cocos nucifera, conservation biologique, congélation, encapsulation, déshydratation, plumule, tissu végétal, saccharose, régénération in vitro, pousse, cryoconservation, http://aims.fao.org/aos/agrovoc/c_1716, http://aims.fao.org/aos/agrovoc/c_27843, http://aims.fao.org/aos/agrovoc/c_3091, http://aims.fao.org/aos/agrovoc/c_28357, http://aims.fao.org/aos/agrovoc/c_2162, http://aims.fao.org/aos/agrovoc/c_6029, http://aims.fao.org/aos/agrovoc/c_27990, http://aims.fao.org/aos/agrovoc/c_7495, http://aims.fao.org/aos/agrovoc/c_34114, http://aims.fao.org/aos/agrovoc/c_14261, http://aims.fao.org/aos/agrovoc/c_24155, http://aims.fao.org/aos/agrovoc/c_4027,
Online Access:	http://agritrop.cirad.fr/546471/ http://agritrop.cirad.fr/546471/1/document_546471.pdf
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