Determination of fish origin by using 16S rDNA fingerprinting of bacterial communities by PCR-DGGE : An application on Pangasius fish from Viet Nam
The determination of geographical origin is a demand of the traceability system of import-export food products. One hypothesis of tracing the source of a product is by analysing in a global way the bacterial communities of the food samples after their exportation. For this purpose, molecular techniques employing 16S rDNA profiles generated by PCR-DGGE were used to detect the variation in bacterial community structures of Pangasius fish from An Giang province, South Viet Nam harvested in different aquaculture farm and during two different seasons, the rainy season and the dry season. When the 16S rDNA profiles were analysed by multivariate analysis, distinct microbial communities were detected. The band profiles of the fish bacteria from different farms are different and are specific for each location and could be used as a bar code to certify the origin of the fish. When band profiles within the same location at different seasons were compared, we also observed the difference banding pattern for each season. Some common bands were noted which are stable throughout the seasons. These bands can be used as specific markers for this specific location. This method is a new traceability tool which provides fish products with a unique bar code and makes it possible to trace back the fish to their original location.
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Subjects: | Q01 - Sciences et technologies alimentaires - Considérations générales, Q02 - Traitement et conservation des produits alimentaires, Pangasius, provenance, identification, empreinte ADN, PCR, électrophorèse, flore microbienne, analyse microbiologique, http://aims.fao.org/aos/agrovoc/c_24779, http://aims.fao.org/aos/agrovoc/c_16022, http://aims.fao.org/aos/agrovoc/c_3791, http://aims.fao.org/aos/agrovoc/c_34258, http://aims.fao.org/aos/agrovoc/c_34079, http://aims.fao.org/aos/agrovoc/c_2523, http://aims.fao.org/aos/agrovoc/c_16367, http://aims.fao.org/aos/agrovoc/c_25302, http://aims.fao.org/aos/agrovoc/c_8227, |
Online Access: | http://agritrop.cirad.fr/543976/ http://agritrop.cirad.fr/543976/1/document_543976.pdf |
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dig-cirad-fr-5439762024-01-28T15:48:35Z http://agritrop.cirad.fr/543976/ http://agritrop.cirad.fr/543976/ Determination of fish origin by using 16S rDNA fingerprinting of bacterial communities by PCR-DGGE : An application on Pangasius fish from Viet Nam. Le N'Guyen Doan Duy, Hanh Ha Ngoc, Dijoux Daniel, Loiseau Gérard, Montet Didier. 2008. Food Control, 19 (5) : 454-460.https://doi.org/10.1016/j.foodcont.2007.05.006 <https://doi.org/10.1016/j.foodcont.2007.05.006> Determination of fish origin by using 16S rDNA fingerprinting of bacterial communities by PCR-DGGE : An application on Pangasius fish from Viet Nam Le N'Guyen, Doan Duy Hanh Ha Ngoc, Dijoux, Daniel Loiseau, Gérard Montet, Didier eng 2008 Food Control Q01 - Sciences et technologies alimentaires - Considérations générales Q02 - Traitement et conservation des produits alimentaires Pangasius provenance identification empreinte ADN PCR électrophorèse flore microbienne analyse microbiologique http://aims.fao.org/aos/agrovoc/c_24779 http://aims.fao.org/aos/agrovoc/c_16022 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_34258 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_2523 http://aims.fao.org/aos/agrovoc/c_16367 http://aims.fao.org/aos/agrovoc/c_25302 Viet Nam http://aims.fao.org/aos/agrovoc/c_8227 The determination of geographical origin is a demand of the traceability system of import-export food products. One hypothesis of tracing the source of a product is by analysing in a global way the bacterial communities of the food samples after their exportation. For this purpose, molecular techniques employing 16S rDNA profiles generated by PCR-DGGE were used to detect the variation in bacterial community structures of Pangasius fish from An Giang province, South Viet Nam harvested in different aquaculture farm and during two different seasons, the rainy season and the dry season. When the 16S rDNA profiles were analysed by multivariate analysis, distinct microbial communities were detected. The band profiles of the fish bacteria from different farms are different and are specific for each location and could be used as a bar code to certify the origin of the fish. When band profiles within the same location at different seasons were compared, we also observed the difference banding pattern for each season. Some common bands were noted which are stable throughout the seasons. These bands can be used as specific markers for this specific location. This method is a new traceability tool which provides fish products with a unique bar code and makes it possible to trace back the fish to their original location. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/543976/1/document_543976.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.foodcont.2007.05.006 10.1016/j.foodcont.2007.05.006 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodcont.2007.05.006 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.foodcont.2007.05.006 |
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Q01 - Sciences et technologies alimentaires - Considérations générales Q02 - Traitement et conservation des produits alimentaires Pangasius provenance identification empreinte ADN PCR électrophorèse flore microbienne analyse microbiologique http://aims.fao.org/aos/agrovoc/c_24779 http://aims.fao.org/aos/agrovoc/c_16022 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_34258 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_2523 http://aims.fao.org/aos/agrovoc/c_16367 http://aims.fao.org/aos/agrovoc/c_25302 http://aims.fao.org/aos/agrovoc/c_8227 Q01 - Sciences et technologies alimentaires - Considérations générales Q02 - Traitement et conservation des produits alimentaires Pangasius provenance identification empreinte ADN PCR électrophorèse flore microbienne analyse microbiologique http://aims.fao.org/aos/agrovoc/c_24779 http://aims.fao.org/aos/agrovoc/c_16022 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_34258 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_2523 http://aims.fao.org/aos/agrovoc/c_16367 http://aims.fao.org/aos/agrovoc/c_25302 http://aims.fao.org/aos/agrovoc/c_8227 |
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Q01 - Sciences et technologies alimentaires - Considérations générales Q02 - Traitement et conservation des produits alimentaires Pangasius provenance identification empreinte ADN PCR électrophorèse flore microbienne analyse microbiologique http://aims.fao.org/aos/agrovoc/c_24779 http://aims.fao.org/aos/agrovoc/c_16022 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_34258 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_2523 http://aims.fao.org/aos/agrovoc/c_16367 http://aims.fao.org/aos/agrovoc/c_25302 http://aims.fao.org/aos/agrovoc/c_8227 Q01 - Sciences et technologies alimentaires - Considérations générales Q02 - Traitement et conservation des produits alimentaires Pangasius provenance identification empreinte ADN PCR électrophorèse flore microbienne analyse microbiologique http://aims.fao.org/aos/agrovoc/c_24779 http://aims.fao.org/aos/agrovoc/c_16022 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_34258 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_2523 http://aims.fao.org/aos/agrovoc/c_16367 http://aims.fao.org/aos/agrovoc/c_25302 http://aims.fao.org/aos/agrovoc/c_8227 Le N'Guyen, Doan Duy Hanh Ha Ngoc, Dijoux, Daniel Loiseau, Gérard Montet, Didier Determination of fish origin by using 16S rDNA fingerprinting of bacterial communities by PCR-DGGE : An application on Pangasius fish from Viet Nam |
description |
The determination of geographical origin is a demand of the traceability system of import-export food products. One hypothesis of tracing the source of a product is by analysing in a global way the bacterial communities of the food samples after their exportation. For this purpose, molecular techniques employing 16S rDNA profiles generated by PCR-DGGE were used to detect the variation in bacterial community structures of Pangasius fish from An Giang province, South Viet Nam harvested in different aquaculture farm and during two different seasons, the rainy season and the dry season. When the 16S rDNA profiles were analysed by multivariate analysis, distinct microbial communities were detected. The band profiles of the fish bacteria from different farms are different and are specific for each location and could be used as a bar code to certify the origin of the fish. When band profiles within the same location at different seasons were compared, we also observed the difference banding pattern for each season. Some common bands were noted which are stable throughout the seasons. These bands can be used as specific markers for this specific location. This method is a new traceability tool which provides fish products with a unique bar code and makes it possible to trace back the fish to their original location. |
format |
article |
topic_facet |
Q01 - Sciences et technologies alimentaires - Considérations générales Q02 - Traitement et conservation des produits alimentaires Pangasius provenance identification empreinte ADN PCR électrophorèse flore microbienne analyse microbiologique http://aims.fao.org/aos/agrovoc/c_24779 http://aims.fao.org/aos/agrovoc/c_16022 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_34258 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_2523 http://aims.fao.org/aos/agrovoc/c_16367 http://aims.fao.org/aos/agrovoc/c_25302 http://aims.fao.org/aos/agrovoc/c_8227 |
author |
Le N'Guyen, Doan Duy Hanh Ha Ngoc, Dijoux, Daniel Loiseau, Gérard Montet, Didier |
author_facet |
Le N'Guyen, Doan Duy Hanh Ha Ngoc, Dijoux, Daniel Loiseau, Gérard Montet, Didier |
author_sort |
Le N'Guyen, Doan Duy |
title |
Determination of fish origin by using 16S rDNA fingerprinting of bacterial communities by PCR-DGGE : An application on Pangasius fish from Viet Nam |
title_short |
Determination of fish origin by using 16S rDNA fingerprinting of bacterial communities by PCR-DGGE : An application on Pangasius fish from Viet Nam |
title_full |
Determination of fish origin by using 16S rDNA fingerprinting of bacterial communities by PCR-DGGE : An application on Pangasius fish from Viet Nam |
title_fullStr |
Determination of fish origin by using 16S rDNA fingerprinting of bacterial communities by PCR-DGGE : An application on Pangasius fish from Viet Nam |
title_full_unstemmed |
Determination of fish origin by using 16S rDNA fingerprinting of bacterial communities by PCR-DGGE : An application on Pangasius fish from Viet Nam |
title_sort |
determination of fish origin by using 16s rdna fingerprinting of bacterial communities by pcr-dgge : an application on pangasius fish from viet nam |
url |
http://agritrop.cirad.fr/543976/ http://agritrop.cirad.fr/543976/1/document_543976.pdf |
work_keys_str_mv |
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