Molecular analysis of a full-length sequence of a new yam badnavirus from Dioscorea sansibarensis
Badnavirus-like particles were observed by ISEM in viral preparations from yam (Dioscorea sansibarensis) leaves from Benin. Use of the viral preparation as template for PCR amplification with badnavirus-specific primers gave rise to a 579-bp product with most nucleotide identity (70.8%) to Dioscorea alata bacilliform virus (DaBV, Accession numbers X94575-X94582), the only other yam badnavirus sequenced to date. A full-length badnavirus sequence was generated, which consisted of 7261 nucleotides with a typical Badnavirus genome organisation. The full-length sequence shared most identity (61.9%) to DaBV (Accession numbers X94575-X94582) and hence represents a member of a new badnavirus species termed Dioscorea sansibarensis bacilliform virus (DsBV).
| Main Authors: | , |
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| Format: | article biblioteca |
| Language: | eng |
| Published: |
Springer
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| Subjects: | H20 - Maladies des plantes, U30 - Méthodes de recherche, Dioscorea, virus des végétaux, identification, PCR, séquence nucléotidique, http://aims.fao.org/aos/agrovoc/c_2288, http://aims.fao.org/aos/agrovoc/c_5985, http://aims.fao.org/aos/agrovoc/c_3791, http://aims.fao.org/aos/agrovoc/c_34079, http://aims.fao.org/aos/agrovoc/c_27583, |
| Online Access: | http://agritrop.cirad.fr/542534/ http://agritrop.cirad.fr/542534/1/542534.pdf |
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