Recombinant capriposviruses expressing proteins of bluetongue virus : evaluation of immune response and protection in small ruminants

Bluetongue is an infectious arthropod-borne viral disease affecting sheep, domestic and wild ruminants caused by Bluetongue Virus (BTV) and transmitted by few species of Culicoides (Diptera: Ceratopogonidae). Since 1998, outbreaks of BT involving 6 distinct serotypes have occurred in the Mediterranean basin. The only BTV vaccines currently available are serotype specific. Objectives: The development of recombinants capripoxvirus for protective immunization of ruminants against bluetongue virus (BTV) infection is described here. Methodology: Sheep (n=11) and goats (n=4) were immunized with bluetongue recombinant capripoxvirus (BTV-Cpox) expressing individually four different genes encoding two capsid proteins (VP2 and VP7) and two non-structural proteins (NS1, NS3) of Bluetongue virus serotype 2 (BTV-2). Results: Seroconversion was observed against NS3, VP7 and VP2 in both groups of animals. A specific BTV antigens lymphoproliferation observed in goats corroborates with a partial protection in sheep challenged with a virulent strain of BTV-2.

Bibliographic Details

Main Authors:	Perrin, Aurélie, Albina, Emmanuel, Breard, Emmanuel, Sailleau, Corinne, Prome, Sylvie, Grillet, Colette, Kwiatek, Olivier, Russo, Pierre, Thiéry, Richard, Zientara, Stéphan, Cetre-Sossah, Catherine
Format:	conference_item biblioteca
Language:	eng
Published:	CIRAD
Subjects:	L73 - Maladies des animaux,
Online Access:	http://agritrop.cirad.fr/541570/ http://agritrop.cirad.fr/541570/1/ID541570.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!