Identificação e caraterização molecular de genes envolvidos no metabolismo de diterpenos especificos do cafeeiro

The coffee lipids are important components of aromatic and flavor characteristics of the coffee cup. In the lipid fraction, the diterpens cafestol, kahweol and derived compounds (16-O-methylcafestol, 16-O-methylkahweol) are diterpens specific Coffea spp. compounds. Studies have revealed their influence on human health, showing effect on cholesterol level and their action as chemoprotector against toxins with carcinogenic action. In order to increase our knowledge about the genes involved on the coffee diterpens biosynthesis a in silico and in vivo study of the genes involved in the first stages of their pathway (i.e copalyl diphosphate synthase (CPS), kaurene synthase (KS) and kaurene oxidase (KO)) was performed. For gene expression analysis fruits of Coffea arabica cv. IAPAR 59 and Coffea canephora cv. Apoatã at different stages of maturation was used. In silico studies allowed the identification of one gene for CPS and KS and two for KO. It also revealed different expression patterns specific to each gene in terms of level and timing of expression. Results of expression in vivo were slightly different from the ones obtained in silico, and the comparison of C. canephora and C. arabica also revealed some differences. These data will be confirmed by RT-PCR and a whole transcriptome approach using macroarrays based on the Genoma Café Project will be initiated to identify other genes involved in this bisoynthesis pathway.

Bibliographic Details

Main Authors:	Ferreira, Lucia Pires, Plener, Laure, Marraccini, Pierre, Perreira, Luiz Filipe Protasio, Vieira, Luiz Gonzaga Esteves, Pot, David
Format:	conference_item biblioteca
Language:	por
Published:	s.n.
Subjects:	F30 - Génétique et amélioration des plantes,
Online Access:	http://agritrop.cirad.fr/540472/ http://agritrop.cirad.fr/540472/1/document_540472.pdf
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