In silico and in vitro analysis of the isoprenoid pathway in coffee

The most important lipids in coffee, the diterpenes khaweol and cafestol, are originated from the isoprenoid pathway. Despite their diversity in functions and structures, all isoprenoids derive from the common-five carbon building unit isopentenyl diphosphate (IPP) and its isomer dimethylallyl diphosphate (DMAPP). In higher plants, there are two independent pathways located in the cytosol (mevalonic acid or MVA pathway) and in the plastids (methylerythritol phosphate - MEP - or non mevalonic pathway). Throughout the data mining of the Brazilian Coffee Genome Project we studied the genes that code for the enzymes 3-hydroxy-3-methyglutaryl-CoA reductase (HMGR) and mevalonate diphosphate decarboxylase (MPDC) for the MVA pathway and 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) and isopentenyl diphosphate/dimethylallyl diphosphate synthase (IDS) for the MEP pathway.

Bibliographic Details

Main Authors:	Tiski, Iris, Perreira, Luiz Filipe Protasio, Vieira, Luiz Gonzaga Esteves, Takahashi, E.K., Ferreira, Lucia Pires, Marraccini, Pierre, Pot, David, Leroy, Thierry, Benassi, Marta T., Dias, Rafael Carlos Eloy
Format:	conference_item biblioteca
Language:	eng
Published:	ASIC
Subjects:	F30 - Génétique et amélioration des plantes, F60 - Physiologie et biochimie végétale, Coffea, fève de café, isoprénoïde, diterpénoïde, métabolisme des lipides, gène, activité enzymatique, oxydoréductase, lyase, http://aims.fao.org/aos/agrovoc/c_1720, http://aims.fao.org/aos/agrovoc/c_28379, http://aims.fao.org/aos/agrovoc/c_36399, http://aims.fao.org/aos/agrovoc/c_32428, http://aims.fao.org/aos/agrovoc/c_4361, http://aims.fao.org/aos/agrovoc/c_3214, http://aims.fao.org/aos/agrovoc/c_2604, http://aims.fao.org/aos/agrovoc/c_5474, http://aims.fao.org/aos/agrovoc/c_4473,
Online Access:	http://agritrop.cirad.fr/540078/ http://agritrop.cirad.fr/540078/1/ID540078.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!