Cross-amplification tests of ungulate primers in roe deer (Capreolus capreolus) to develop a multiplex panel of 12 microsatellite loci
Cross-amplification permits transposition of microsatellite markers to closely related species. Multiplexing reduces time and cost further, exploiting simultaneous amplification of several primers. In cross-amplification tests of 55 ungulate primers in roe deer, 39 gave specific amplification products and 20 were polymorphic. Twelve primers were retained to form a multiplex kit. In 30 roe deer, average allelic diversity was 5.67 (range: 2-15), expected heterozygosity was 0.664 and mean polymorphic information content (PIC) value was 0.605. Probability of identity was P ID = 5 X 10-11 and probability of exclusion for parentage studies was P E1 = 0.98856 and P E2 = 0.99952.
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| Main Authors: | , , , , , |
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| Format: | article biblioteca |
| Language: | eng |
| Subjects: | L50 - Physiologie et biochimie animales, Bovidae, Cervidae, PCR, microsatellite, chevreuil, http://aims.fao.org/aos/agrovoc/c_1038, http://aims.fao.org/aos/agrovoc/c_1480, http://aims.fao.org/aos/agrovoc/c_34079, http://aims.fao.org/aos/agrovoc/c_36574, http://aims.fao.org/aos/agrovoc/c_24107, |
| Online Access: | http://agritrop.cirad.fr/538220/ http://agritrop.cirad.fr/538220/1/document_538220.pdf |
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