A protocol to analyse cellular dynamics during plant development

In vivo microscopy generates images that contain complex information on the dynamic behaviour of three-dimensional (3D) objects. As a result, adapted mathematical and computational tools are required to help in their interpretation. Ideally, a complete software chain to study the dynamics of a complex 3D object should include: (i) the acquisition, (ii) the preprocessing and (iii) segmentation of the images, followed by (iv) a reconstruction in time and space and (v) the final quantitative analysis. Here, we have developed such a protocol to study cell dynamics at the shoot apical meristem in Arabidopsis. The protocol uses serial optical sections made with the confocal microscope. It includes specially designed algorithms to automate the identification of cell lineage and to analyse the quantitative behaviour of the meristem surface.

Bibliographic Details

Main Authors:	Barbier de Reuille, Pierre, Bohn-Courseau, Isabelle, Godin, Christophe, Traas, Jan
Format:	article biblioteca
Language:	eng
Published:	Wiley
Subjects:	F62 - Physiologie végétale - Croissance et développement, U10 - Informatique, mathématiques et statistiques, analyse d'image, imagerie, Arabidopsis thaliana, méristème apical, développement biologique, technique analytique, microscopie, cellule, identification, reconstitution, modèle mathématique, http://aims.fao.org/aos/agrovoc/c_36762, http://aims.fao.org/aos/agrovoc/c_36760, http://aims.fao.org/aos/agrovoc/c_33292, http://aims.fao.org/aos/agrovoc/c_528, http://aims.fao.org/aos/agrovoc/c_921, http://aims.fao.org/aos/agrovoc/c_1513, http://aims.fao.org/aos/agrovoc/c_4810, http://aims.fao.org/aos/agrovoc/c_1418, http://aims.fao.org/aos/agrovoc/c_3791, http://aims.fao.org/aos/agrovoc/c_6476, http://aims.fao.org/aos/agrovoc/c_24199,
Online Access:	http://agritrop.cirad.fr/530277/ http://agritrop.cirad.fr/530277/1/document_530277.pdf
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