Laboratory evaluation of feeding deterrent from root extracts of Mucuna pruriens (Fabaceae) against the desert and the migratory locusts
Crude ethanolic root- extracts of Mucuna pruriens (Fabaceae), a wild plant traditionally used in western Sudan for its pesticide properties, were tested under nochoice condition against the Migratory Locust, Locusta migratoria L. and the Desert Locust, Schistocerca gregaria Forskal (Orthoptera: Acrididae). In this test, locusts were used to investigate the feeding inhibitory action of the extracts of Mucuna. This work was carried out at CIRAD-Prifas Laboratories in Montpellier, France. The trials were conducted on 7+1 day-old adults of each of the two locust species separately. A piece of filter paper 1x2 cm in dia. was submerged for 30 sec. in 10% sugar solution, allowed to dry, and then held on crocodile clip fixed in a test tube plastic stopper. Sugar was used to stimulate feeding. Volumes of 20ul of Mucuna extracts and increased up to 100g/l were applied on the filter paper in the form of droplets by means a Packard microapplicator fitted with glass micro syringe of l ml capacity. The treated filter paper was dried under room conditions for one hour. The locust in the test tube has access to feed, but not to walk in the filter paper. Each test was conducted on 30-33 individuals; each individual was considered as a replicate. Data were collected on the basis of acceptance or rejection of feeding on the filter paper every hour for the first 6h, then after 24h. and 48h. Equivalent number of replicates were made for the respective locusts to serve as control by applying a 20ul of the solvent on the filter paper. The tested extracts showed more antifeedant effects on the Migratory Locust than on the Desert Locust. (Texte intégral)
| Main Authors: | , , , |
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| Format: | conference_item biblioteca |
| Language: | eng |
| Published: |
s.n.
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| Subjects: | H10 - Ravageurs des plantes, Locusta migratoria, Schistocerca gregaria, Mucuna pruriens, lutte anti-insecte, lutte biologique, insecticide d'origine végétale, phagorépulsif, expérimentation en laboratoire, http://aims.fao.org/aos/agrovoc/c_26554, http://aims.fao.org/aos/agrovoc/c_31931, http://aims.fao.org/aos/agrovoc/c_4971, http://aims.fao.org/aos/agrovoc/c_3885, http://aims.fao.org/aos/agrovoc/c_918, http://aims.fao.org/aos/agrovoc/c_1021, http://aims.fao.org/aos/agrovoc/c_16159, http://aims.fao.org/aos/agrovoc/c_15989, |
| Online Access: | http://agritrop.cirad.fr/526053/ |
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