Identification of a DNA marker associated with dwarf trait in Hevea brasiliensis (Muell.) Arg. through random amplified polymorphic DNA analysis
The current study was conducted to identify DNA markers linked to genes controlling dwarf character in rubber tree (Hevea brasiliensis) using random amplified polymorphic DNA (RAPD) analysis. Hybrid progenies were developed between dwarf parent (natural variant) and cultivated clone RRII 118. Genomic DNA was subjected to PCR analysis using 85 random sequence 10-mer primers, of which 9 produced clear, scorable bands. Among the 9, one showed polymorphism which was used for dwarf character analysis. An initial comparison was made between two parents and later the dwarf parent along with their hybrid progenies. In the dwarf parent alone with the primer OPB-12, a DNA fragment with molecular size of 1.3 kb was amplified. This primer amplified the same DNA fragment (1.3 kb) with the segregated 6 F1 hybrid progenies and it was absent in the other parent. The segregation data for the marker and the dwarf trait in the F1 hybrids indicate that the DNA marker identified is linked to dwarf character. Analysis of RAPD loci by southern blot hybridization indicated that this is a specific loci of the same gene fragment. DNA markers associated with dwarf trait genes are useful to facilitate the introgression of these genes in breeding materials for the development of high yielding clones with dwarf nature to avoid wind damage of this important perennial tree crop.
| Main Authors: | , , , , |
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| Format: | conference_item biblioteca |
| Language: | eng |
| Published: |
CIRAD
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| Subjects: | F30 - Génétique et amélioration des plantes, U30 - Méthodes de recherche, |
| Online Access: | http://agritrop.cirad.fr/513732/ http://agritrop.cirad.fr/513732/1/ID513732.pdf |
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