Transgenic Bt rice expressing a synthetic cry 1B gene : expression strategies and field protection against the striped stem borer : expression strategies and field protection against the striped stem borer
We investigated whether the conditional, wound-inducible production of a Bacillus thuringiensis (Bt) Cry endotoxin may provide a hopeful and efficient alternative of protection from stem borer damage to the high dosage, constitutive or tissue-specific toxin delivery approach generally used in transgenic Bt crops. A synthetic cry1B gene controlled by the promoter region of either the constitutive ubiquitin (ubi) gene or of a wound-inducible proteinase inhibitor (mpi) gene from maize was introduced in rice by microprojectile bombardment. Accumulation of Cry1B from undetectable level to 0.2% of total soluble protein, 8-12 hours after mechanical wounding, was demonstrated in leaf and pith tissues of lines harbouring the pmpi-cry1B construct. In these plants, Cry1B was not detected in pollen and seed endosperm and embryo, unlike in pUbi-cry1B plants. Two out of 43 pmpi-cry1B plants were found to exhibit full protection against SSB damage. Field evaluation with artificial and natural infestations of the most promising ubi-cry1B and mpi-cry1B lines in Delta Del Ebre, Spain during the summer crop season 2001 confirmed the level of protection observed in greenhouse assays.
| Main Authors: | , , , , , , , , , |
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| Format: | conference_item biblioteca |
| Language: | eng |
| Published: |
SIP
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| Subjects: | H10 - Ravageurs des plantes, Chilo suppressalis, lutte antiravageur, Oryza, plante transgénique, gène, Bacillus thuringiensis, expérimentation au champ, http://aims.fao.org/aos/agrovoc/c_27157, http://aims.fao.org/aos/agrovoc/c_5726, http://aims.fao.org/aos/agrovoc/c_5435, http://aims.fao.org/aos/agrovoc/c_27619, http://aims.fao.org/aos/agrovoc/c_3214, http://aims.fao.org/aos/agrovoc/c_761, http://aims.fao.org/aos/agrovoc/c_33990, |
| Online Access: | http://agritrop.cirad.fr/510411/ |
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