Molecular approaches to blast and brown spot in rice (Oryza sativa L.) of Nortern Italy

Blast, caused by Pyricularia grisea, is one of the most widespread and serious diseases of rice. The fungus is highly variable with respect to its infectivity. In order to define the Italian P. grisea population with respect to this variability, 45 different strains were analysed using rep-PCR (repetitive-element-based polymerase chain reaction). Statistical analysis of the results allowed the identification of 9 different lineages. Symptoms of brown spot (caused by Bipolaris oryzae), another disease of worldwide occurrence, are frequently mistaken with those of blast infection. PCR-based methods to distinguish brown spot from rice blast disease were developed. Preventive spraying with fungicides against blast is widely practiced in Italy; the use of these molecular methods to distinguish between the two diseases could lead to a more rational use of chemicals.

Bibliographic Details

Main Authors:	Piotti, Eleonora, Rodino, Doriana, Rigano, M.M., Rodolfi, M., Castiglione, S., Biloni, Massimo, Picco, A.M., Sala, Francesco
Format:	conference_item biblioteca
Language:	eng
Published:	CIRAD
Subjects:	H20 - Maladies des plantes, Oryza sativa, maladie des plantes, Pyricularia, variation génétique, Bipolaris, identification, maladie fongique, PCR, diagnostic, maladie de la pyriculariose des plantes, http://aims.fao.org/aos/agrovoc/c_5438, http://aims.fao.org/aos/agrovoc/c_5962, http://aims.fao.org/aos/agrovoc/c_16024, http://aims.fao.org/aos/agrovoc/c_15975, http://aims.fao.org/aos/agrovoc/c_31546, http://aims.fao.org/aos/agrovoc/c_3791, http://aims.fao.org/aos/agrovoc/c_11042, http://aims.fao.org/aos/agrovoc/c_34079, http://aims.fao.org/aos/agrovoc/c_2238, http://aims.fao.org/aos/agrovoc/c_9312, http://aims.fao.org/aos/agrovoc/c_4026,
Online Access:	http://agritrop.cirad.fr/510152/
Tags:	Add Tag No Tags, Be the first to tag this record!