Histo-cytological study of apices of coffee (Coffea racemosa and C. sessiliflora) in vitro plantlets during their cryopreservation using the encapsulation-dehydration technique
Apices of in vitro plantlets of Coffea racemosa and C. sessiliflora withstood freezing in liquid nitrogen using the encapsulation-dehydration technique. An intense intracellular starch accumulation was noted after overnight culture of apices on standard medium. Apices of C. sessiliflora required a 3 to 10-d pregrowth in liquid medium containing 0.75 M sucrose, whereas those of C. racemosa required a progressive increase of the sucrose concentration from 0.5 to 1 M. After the dehydration phase, cells of some apices were severely damaged, showing osmiophilic granules on the surface of the plasmalemma. In other apices, cells were less severely damaged, showing exocytosis vesicles at the periphery of the plasma membrane. After freezing, survival rates of 27 and 37% were obtained for C. racemosa and C. sessiliflora, respectively. Two growth recovery patterns were observed: direct development of foliar primordia and callusing.
| Main Authors: | , , , , |
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| Format: | article biblioteca |
| Language: | eng |
| Subjects: | F02 - Multiplication végétative des plantes, F60 - Physiologie et biochimie végétale, Coffea, culture de méristème, pousse, congélation, encapsulation, séchage osmotique, analyse histocytologique, Coffea racemosa, http://aims.fao.org/aos/agrovoc/c_1720, http://aims.fao.org/aos/agrovoc/c_7043, http://aims.fao.org/aos/agrovoc/c_14261, http://aims.fao.org/aos/agrovoc/c_3091, http://aims.fao.org/aos/agrovoc/c_28357, http://aims.fao.org/aos/agrovoc/c_36939, http://aims.fao.org/aos/agrovoc/c_27541, http://aims.fao.org/aos/agrovoc/c_1728, |
| Online Access: | http://agritrop.cirad.fr/484987/ |
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