A highly sensitive, non radioactive assay for T cell activation in cattle : applications in scereening for antigens recognised by CD4+ and CD8+ T cells

We describe a highly sensitive, non-radioactive assay for T cell activation, based on the rapid induction of class II MHC expression by constitutively negative bovine endothelial cells, when cultured in the presence of supernatants derived from activated bovine T cells. We demonstrate the effectiveness of this assay in detecting rBoIFNy and activation of immune CD4 + and CD8 + T cell lines and clones in response to specific antigen and transfected COS-7 cells, respectively. We also demonstrate its utility in identifying purified pathogen fractions that activate immune CD4 + T cell clones.

Bibliographic Details

Main Authors:	Ballingall, Keith T., Mwangi, Duncan M., MacHugh, Niall D., Taracha, Evans L.N., Totté, Philippe, McKeever, Declan J.
Format:	article biblioteca
Language:	eng
Subjects:	L50 - Physiologie et biochimie animales, cellule, immunité cellulaire, bovin, antigène, immunologie, http://aims.fao.org/aos/agrovoc/c_1418, http://aims.fao.org/aos/agrovoc/c_9692, http://aims.fao.org/aos/agrovoc/c_1391, http://aims.fao.org/aos/agrovoc/c_504, http://aims.fao.org/aos/agrovoc/c_3808,
Online Access:	http://agritrop.cirad.fr/478228/ http://agritrop.cirad.fr/478228/1/478228.pdf
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