Strategy to study functionality of putative promotor from Hevea brasiliensis. Attemps of Agrobacterium tumefaciens-mediated gene transfer in various tissue

Genetic transformation via #Agrobacterium tumefaciens# was carried out in various plant organs and #in vitro# cell tissue of #Hevea brasiliensis#. Transient expression of the gusA reporter gene was recorded in both tegument calli and longterm friable calli. Other differentiated organs and embryos are not susceptible to the infection with 5 different agrobacteria strains. Northern blot analysis showed that the ethylene-inducible glutamine synthetase GS1 is expressed in friable calli as in latex cells of ethylene-stimulated trees. Therefore, such tissue might be used for the functional analysis of GS1 promoter. On the contrary, Hevein expression is strictly latex cell-specific and we assume that its promotor can be studied only in differentiated latex cell from transgenic rubber tree.

Bibliographic Details

Main Authors:	Montoro, Pascal, Pujade-Renaud, Valérie, Teinseree, Nongluk
Format:	conference_item biblioteca
Language:	eng
Published:	Mahidol University
Subjects:	F30 - Génétique et amélioration des plantes, Hevea brasiliensis, Agrobacterium tumefaciens, transfert de gène, transformation génétique, génie génétique, http://aims.fao.org/aos/agrovoc/c_3589, http://aims.fao.org/aos/agrovoc/c_27444, http://aims.fao.org/aos/agrovoc/c_24846, http://aims.fao.org/aos/agrovoc/c_3220, http://aims.fao.org/aos/agrovoc/c_15974,
Online Access:	http://agritrop.cirad.fr/464993/ http://agritrop.cirad.fr/464993/1/ID464993.pdf
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