Strategy to study functionality of putative promotor from Hevea brasiliensis. Attemps of Agrobacterium tumefaciens-mediated gene transfer in various tissue
Genetic transformation via #Agrobacterium tumefaciens# was carried out in various plant organs and #in vitro# cell tissue of #Hevea brasiliensis#. Transient expression of the gusA reporter gene was recorded in both tegument calli and longterm friable calli. Other differentiated organs and embryos are not susceptible to the infection with 5 different agrobacteria strains. Northern blot analysis showed that the ethylene-inducible glutamine synthetase GS1 is expressed in friable calli as in latex cells of ethylene-stimulated trees. Therefore, such tissue might be used for the functional analysis of GS1 promoter. On the contrary, Hevein expression is strictly latex cell-specific and we assume that its promotor can be studied only in differentiated latex cell from transgenic rubber tree.
| Main Authors: | , , |
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| Format: | conference_item biblioteca |
| Language: | eng |
| Published: |
Mahidol University
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| Subjects: | F30 - Génétique et amélioration des plantes, Hevea brasiliensis, Agrobacterium tumefaciens, transfert de gène, transformation génétique, génie génétique, http://aims.fao.org/aos/agrovoc/c_3589, http://aims.fao.org/aos/agrovoc/c_27444, http://aims.fao.org/aos/agrovoc/c_24846, http://aims.fao.org/aos/agrovoc/c_3220, http://aims.fao.org/aos/agrovoc/c_15974, |
| Online Access: | http://agritrop.cirad.fr/464993/ http://agritrop.cirad.fr/464993/1/ID464993.pdf |
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