Spectrophotometry assay of lipase activity using Rhodamine 6G
A method for lipase activity determination by spectrophotometry is described. Rhodamme 60 reagent is used for complexation with the free fatty ac1ds liberated during lipolysis This method uses toluene as the Rhodamine 6G solvent in the presencc of fatty acids, a pink coloration appears and absorbance is read at 513 nm it is shown this method is pH independent and gives results similar to those obtained with TCL technique. This method is utilized for lipase activity determination in a yeast strain Candida steatolytica. (Résumé
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Format: | article biblioteca |
Language: | eng |
Subjects: | triacylglycérol lipase, activité enzymatique, spectrométrie, levure, http://aims.fao.org/aos/agrovoc/c_24095, http://aims.fao.org/aos/agrovoc/c_2604, http://aims.fao.org/aos/agrovoc/c_7283, http://aims.fao.org/aos/agrovoc/c_8480, |
Online Access: | http://agritrop.cirad.fr/437335/ http://agritrop.cirad.fr/437335/1/ID437335.pdf |
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dig-cirad-fr-4373352024-01-28T04:21:38Z http://agritrop.cirad.fr/437335/ http://agritrop.cirad.fr/437335/ Spectrophotometry assay of lipase activity using Rhodamine 6G. Van Autryve P., Ratomahenina Robert, Riaublanc A., Mitrani C., Pina Michel, Graille Jean, Galzy Pierre. 1991. Oléagineux, 46 (1) : 29-31. Spectrophotometry assay of lipase activity using Rhodamine 6G Van Autryve, P. Ratomahenina, Robert Riaublanc, A. Mitrani, C. Pina, Michel Graille, Jean Galzy, Pierre eng 1991 Oléagineux triacylglycérol lipase activité enzymatique spectrométrie levure http://aims.fao.org/aos/agrovoc/c_24095 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_7283 http://aims.fao.org/aos/agrovoc/c_8480 A method for lipase activity determination by spectrophotometry is described. Rhodamme 60 reagent is used for complexation with the free fatty ac1ds liberated during lipolysis This method uses toluene as the Rhodamine 6G solvent in the presencc of fatty acids, a pink coloration appears and absorbance is read at 513 nm it is shown this method is pH independent and gives results similar to those obtained with TCL technique. This method is utilized for lipase activity determination in a yeast strain Candida steatolytica. (Résumé article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/437335/1/ID437335.pdf text Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html |
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triacylglycérol lipase activité enzymatique spectrométrie levure http://aims.fao.org/aos/agrovoc/c_24095 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_7283 http://aims.fao.org/aos/agrovoc/c_8480 triacylglycérol lipase activité enzymatique spectrométrie levure http://aims.fao.org/aos/agrovoc/c_24095 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_7283 http://aims.fao.org/aos/agrovoc/c_8480 |
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triacylglycérol lipase activité enzymatique spectrométrie levure http://aims.fao.org/aos/agrovoc/c_24095 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_7283 http://aims.fao.org/aos/agrovoc/c_8480 triacylglycérol lipase activité enzymatique spectrométrie levure http://aims.fao.org/aos/agrovoc/c_24095 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_7283 http://aims.fao.org/aos/agrovoc/c_8480 Van Autryve, P. Ratomahenina, Robert Riaublanc, A. Mitrani, C. Pina, Michel Graille, Jean Galzy, Pierre Spectrophotometry assay of lipase activity using Rhodamine 6G |
description |
A method for lipase activity determination by spectrophotometry is described. Rhodamme 60 reagent is used for complexation with the free fatty ac1ds liberated during lipolysis This method uses toluene as the Rhodamine 6G solvent in the presencc of fatty acids, a pink coloration appears and absorbance is read at 513 nm it is shown this method is pH independent and gives results similar to those obtained with TCL technique. This method is utilized for lipase activity determination in a yeast strain Candida steatolytica. (Résumé |
format |
article |
topic_facet |
triacylglycérol lipase activité enzymatique spectrométrie levure http://aims.fao.org/aos/agrovoc/c_24095 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_7283 http://aims.fao.org/aos/agrovoc/c_8480 |
author |
Van Autryve, P. Ratomahenina, Robert Riaublanc, A. Mitrani, C. Pina, Michel Graille, Jean Galzy, Pierre |
author_facet |
Van Autryve, P. Ratomahenina, Robert Riaublanc, A. Mitrani, C. Pina, Michel Graille, Jean Galzy, Pierre |
author_sort |
Van Autryve, P. |
title |
Spectrophotometry assay of lipase activity using Rhodamine 6G |
title_short |
Spectrophotometry assay of lipase activity using Rhodamine 6G |
title_full |
Spectrophotometry assay of lipase activity using Rhodamine 6G |
title_fullStr |
Spectrophotometry assay of lipase activity using Rhodamine 6G |
title_full_unstemmed |
Spectrophotometry assay of lipase activity using Rhodamine 6G |
title_sort |
spectrophotometry assay of lipase activity using rhodamine 6g |
url |
http://agritrop.cirad.fr/437335/ http://agritrop.cirad.fr/437335/1/ID437335.pdf |
work_keys_str_mv |
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1792493325710786560 |