Molecular and biological characterization of three isolates of geminivirus infecting tomato in Guadeloupe
Since 1989, tomato fields in Guadeloupe have been infested by a geminivirus disease. Symptoms are yellow mosaic, chlorotic mottling, leaf rolling and stunting. A survey showed that the disease was present over all the island. Cloning and sequencing of an isolate lead to the identification of a bipartite geminivirus close to potato yellow mosaic virus (PYMV-G) (Polston et al, 1998). In this study, three other virus isolates were collected from tomato in Grande-Terre and on the leeward coast of Basse-Terre and from whiteflies (Bemisia argentifolii) in Grande-Terre. They were characterized biologically (mechanical and vector transmission, host range) and molecularly. PCR primers based on conserved sequences were used to amplify and sequence the intercistronic region and the N-terminal region of the coat protein gene (CP). Sequence comparisons between these isolates and the clone of PYMV-G were established in order to confirm the occurrence of PYMV in Guadeloupe.
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Subjects: | H20 - Maladies des plantes, Geminiviridae, identification, Solanum, http://aims.fao.org/aos/agrovoc/c_32859, http://aims.fao.org/aos/agrovoc/c_3791, http://aims.fao.org/aos/agrovoc/c_7216, http://aims.fao.org/aos/agrovoc/c_3406, http://aims.fao.org/aos/agrovoc/c_3081, |
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dig-cirad-fr-4012192024-01-28T01:13:46Z http://agritrop.cirad.fr/401219/ http://agritrop.cirad.fr/401219/ Molecular and biological characterization of three isolates of geminivirus infecting tomato in Guadeloupe. Urbino Cica, Gérion A., Caruana Marie-Line. 1998. In : 2nd international workshop on Bemisia and geminiviral diseases. Program and abstracts = [Deuxième séminaire international sur les maladies à Bemisia et gemini virus. Programme et résumés]. Mayer R.T. (ed.), Maxwell D.P. (ed.). s.l. : s.n., 36. International Workshop on Bemisia and Geminiviral Diseases. 2, San Juan, Porto Rico, 7 Juin 1998/12 Juin 1998. Molecular and biological characterization of three isolates of geminivirus infecting tomato in Guadeloupe Urbino, Cica Gérion, A. Caruana, Marie-Line eng 1998 s.n. 2nd international workshop on Bemisia and geminiviral diseases. Program and abstracts = [Deuxième séminaire international sur les maladies à Bemisia et gemini virus. Programme et résumés] H20 - Maladies des plantes Geminiviridae identification Solanum http://aims.fao.org/aos/agrovoc/c_32859 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_7216 Guadeloupe France http://aims.fao.org/aos/agrovoc/c_3406 http://aims.fao.org/aos/agrovoc/c_3081 Since 1989, tomato fields in Guadeloupe have been infested by a geminivirus disease. Symptoms are yellow mosaic, chlorotic mottling, leaf rolling and stunting. A survey showed that the disease was present over all the island. Cloning and sequencing of an isolate lead to the identification of a bipartite geminivirus close to potato yellow mosaic virus (PYMV-G) (Polston et al, 1998). In this study, three other virus isolates were collected from tomato in Grande-Terre and on the leeward coast of Basse-Terre and from whiteflies (Bemisia argentifolii) in Grande-Terre. They were characterized biologically (mechanical and vector transmission, host range) and molecularly. PCR primers based on conserved sequences were used to amplify and sequence the intercistronic region and the N-terminal region of the coat protein gene (CP). Sequence comparisons between these isolates and the clone of PYMV-G were established in order to confirm the occurrence of PYMV in Guadeloupe. conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/closedAccess http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=98671 |
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H20 - Maladies des plantes Geminiviridae identification Solanum http://aims.fao.org/aos/agrovoc/c_32859 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_7216 http://aims.fao.org/aos/agrovoc/c_3406 http://aims.fao.org/aos/agrovoc/c_3081 H20 - Maladies des plantes Geminiviridae identification Solanum http://aims.fao.org/aos/agrovoc/c_32859 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_7216 http://aims.fao.org/aos/agrovoc/c_3406 http://aims.fao.org/aos/agrovoc/c_3081 |
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H20 - Maladies des plantes Geminiviridae identification Solanum http://aims.fao.org/aos/agrovoc/c_32859 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_7216 http://aims.fao.org/aos/agrovoc/c_3406 http://aims.fao.org/aos/agrovoc/c_3081 H20 - Maladies des plantes Geminiviridae identification Solanum http://aims.fao.org/aos/agrovoc/c_32859 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_7216 http://aims.fao.org/aos/agrovoc/c_3406 http://aims.fao.org/aos/agrovoc/c_3081 Urbino, Cica Gérion, A. Caruana, Marie-Line Molecular and biological characterization of three isolates of geminivirus infecting tomato in Guadeloupe |
description |
Since 1989, tomato fields in Guadeloupe have been infested by a geminivirus disease. Symptoms are yellow mosaic, chlorotic mottling, leaf rolling and stunting. A survey showed that the disease was present over all the island. Cloning and sequencing of an isolate lead to the identification of a bipartite geminivirus close to potato yellow mosaic virus (PYMV-G) (Polston et al, 1998). In this study, three other virus isolates were collected from tomato in Grande-Terre and on the leeward coast of Basse-Terre and from whiteflies (Bemisia argentifolii) in Grande-Terre. They were characterized biologically (mechanical and vector transmission, host range) and molecularly. PCR primers based on conserved sequences were used to amplify and sequence the intercistronic region and the N-terminal region of the coat protein gene (CP). Sequence comparisons between these isolates and the clone of PYMV-G were established in order to confirm the occurrence of PYMV in Guadeloupe. |
format |
conference_item |
topic_facet |
H20 - Maladies des plantes Geminiviridae identification Solanum http://aims.fao.org/aos/agrovoc/c_32859 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_7216 http://aims.fao.org/aos/agrovoc/c_3406 http://aims.fao.org/aos/agrovoc/c_3081 |
author |
Urbino, Cica Gérion, A. Caruana, Marie-Line |
author_facet |
Urbino, Cica Gérion, A. Caruana, Marie-Line |
author_sort |
Urbino, Cica |
title |
Molecular and biological characterization of three isolates of geminivirus infecting tomato in Guadeloupe |
title_short |
Molecular and biological characterization of three isolates of geminivirus infecting tomato in Guadeloupe |
title_full |
Molecular and biological characterization of three isolates of geminivirus infecting tomato in Guadeloupe |
title_fullStr |
Molecular and biological characterization of three isolates of geminivirus infecting tomato in Guadeloupe |
title_full_unstemmed |
Molecular and biological characterization of three isolates of geminivirus infecting tomato in Guadeloupe |
title_sort |
molecular and biological characterization of three isolates of geminivirus infecting tomato in guadeloupe |
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url |
http://agritrop.cirad.fr/401219/ |
work_keys_str_mv |
AT urbinocica molecularandbiologicalcharacterizationofthreeisolatesofgeminivirusinfectingtomatoinguadeloupe AT geriona molecularandbiologicalcharacterizationofthreeisolatesofgeminivirusinfectingtomatoinguadeloupe AT caruanamarieline molecularandbiologicalcharacterizationofthreeisolatesofgeminivirusinfectingtomatoinguadeloupe |
_version_ |
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