Molecular and biological characterization of three isolates of geminivirus infecting tomato in Guadeloupe
Since 1989, tomato fields in Guadeloupe have been infested by a geminivirus disease. Symptoms are yellow mosaic, chlorotic mottling, leaf rolling and stunting. A survey showed that the disease was present over all the island. Cloning and sequencing of an isolate lead to the identification of a bipartite geminivirus close to potato yellow mosaic virus (PYMV-G) (Polston et al, 1998). In this study, three other virus isolates were collected from tomato in Grande-Terre and on the leeward coast of Basse-Terre and from whiteflies (Bemisia argentifolii) in Grande-Terre. They were characterized biologically (mechanical and vector transmission, host range) and molecularly. PCR primers based on conserved sequences were used to amplify and sequence the intercistronic region and the N-terminal region of the coat protein gene (CP). Sequence comparisons between these isolates and the clone of PYMV-G were established in order to confirm the occurrence of PYMV in Guadeloupe.
| Main Authors: | , , |
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| Format: | conference_item biblioteca |
| Language: | eng |
| Published: |
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| Subjects: | H20 - Maladies des plantes, Geminiviridae, identification, Solanum, http://aims.fao.org/aos/agrovoc/c_32859, http://aims.fao.org/aos/agrovoc/c_3791, http://aims.fao.org/aos/agrovoc/c_7216, http://aims.fao.org/aos/agrovoc/c_3406, http://aims.fao.org/aos/agrovoc/c_3081, |
| Online Access: | http://agritrop.cirad.fr/401219/ |
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