In vitro shoot apex micrografting of mature Acacia mangium
Prospects for in vitro micrografting shoot apices of masure Acacia mangium trees were investigated with the use of 432 micrografts. Overal1 success rates of 51.5% were obtained for shoot apices ranging from 200 to 400 um in length, and a short basai wedge of underlying tissues top-grafted in aseptic conditions onto 2-to-3-month-old in vitro grown Acacia mangium seedlings. The successfully established micrografts displayed, however, substantiel variability in terms of further scion elongation as 41% of these micrografts, or 21.2% only of the total amount of the micrografts performed, had resumed growth two months after micrografting. The elongated scions exhibited different types of morphology, ranging from juvenile-like type composed leaves to the predominant mature-like phyllode morphology. Side-grafting, a more difficult procedure to perform than top-grafting, or placing the micrografts for 2 weeks in darkness after grafting, did not improve the scores. Moreover, attempts to micrograft meristems (150 200 um) resulted in 5% success only.
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| Format: | article biblioteca |
| Language: | eng |
| Subjects: | F02 - Multiplication végétative des plantes, F30 - Génétique et amélioration des plantes, Acacia mangium, greffage, vitroplant, multiplication végétative, anatomie végétale, viabilité, http://aims.fao.org/aos/agrovoc/c_42, http://aims.fao.org/aos/agrovoc/c_3344, http://aims.fao.org/aos/agrovoc/c_27626, http://aims.fao.org/aos/agrovoc/c_8177, http://aims.fao.org/aos/agrovoc/c_5954, http://aims.fao.org/aos/agrovoc/c_8210, http://aims.fao.org/aos/agrovoc/c_7260, |
| Online Access: | http://agritrop.cirad.fr/390171/ http://agritrop.cirad.fr/390171/1/390171.pdf |
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