RAPD-based screening of genomic libraries for positional cloning
RAPD markers are frequently used for positional cloning. However, RAPD markers often contain repeated sequences which prevent genomic library screening by hybridisation. We have developed a simple RAPD analysis of genomic libraries based on the identification of cosmid pools and clones amplifying the RAPD marker of interest. Our method does not require the cloning or characterisation of the RAPD marker as it relies on the analysis of cosmid pools or clones using a simple RAPD protocol. We applied this strategy using four RAPD markers composed of single copy or repeated sequences linked to avirulence genes of the rice blast fungus #Magnaporthe grisea#. Cosmids containing these RAPD markers were easily and rapidly identified allowing the construction of physical contigs at these loci.
Main Authors: | , , |
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Format: | article biblioteca |
Language: | eng |
Subjects: | H20 - Maladies des plantes, F30 - Génétique et amélioration des plantes, Oryza, Magnaporthe, pouvoir pathogène, génome, carte génétique, RAPD, banque de gènes, maladie de la pyriculariose des plantes, http://aims.fao.org/aos/agrovoc/c_5435, http://aims.fao.org/aos/agrovoc/c_31827, http://aims.fao.org/aos/agrovoc/c_5629, http://aims.fao.org/aos/agrovoc/c_3224, http://aims.fao.org/aos/agrovoc/c_24002, http://aims.fao.org/aos/agrovoc/c_36341, http://aims.fao.org/aos/agrovoc/c_11116, http://aims.fao.org/aos/agrovoc/c_9312, |
Online Access: | http://agritrop.cirad.fr/389815/ http://agritrop.cirad.fr/389815/1/389815.pdf |
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