Characteristics and interpretation of the indirect-ELISA for Trypanosoma vivax; proposal for the standardisation of the results
Indirect-ELISA for #T. vivax# is the most sensitive technique for antibody detection of #T. vivax# infections. A group of expert has given recommendations for standardisation of the indirect-ELISA. Based on these recommendations we have added some proposals for standardisation and presentation of the results; the example of the indirect-ELISA for #T. vivax# in cattle is presented. Characteristics of the indirect-ELISA have been studied in experimental conditions in sheep, and in natural infections, in cattle in French Guyana. It is proposed to present the results in relative percentage of positivity " RPP ": percentage of positivity relative to negative and positive reference samples to avoid any variation from plate to plate, or dependant of the activity of the reagants. Reference samples are chosen to be representative of the infected and non infected populations. 300 samples from each population are tested, mean response to the test is recorded and the reference samples selected for a similar response. Such presentation of the results allow to determine sensitivity and specificity of the test when fixing the cut off line either on a graph of the distribution of the responses in infected and non infected populations or, directly with the Gaussian distributions. Characteristics of the test in 13 sheep experimentally infected with #T. vivax# indicated that the RPP of the samples is below 5% before infection, reaches 50% in 12-30 days post infection (p.I.), remains above 30% throughout the infection, and decreases below 30% within 2-3 months after a sterilizing treatment. It is suggested to use RPP for the presentation of the results in ELISA tests, for better standardisation and comparison of the results between laboratories
| Main Authors: | , |
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| Format: | conference_item biblioteca |
| Language: | eng |
| Published: |
IICA
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| Subjects: | L72 - Organismes nuisibles des animaux, L73 - Maladies des animaux, Trypanosoma vivax, Test ELISA, méthode, analyse de données, bovin, ovin, expérimentation, http://aims.fao.org/aos/agrovoc/c_27408, http://aims.fao.org/aos/agrovoc/c_15968, http://aims.fao.org/aos/agrovoc/c_4788, http://aims.fao.org/aos/agrovoc/c_15962, http://aims.fao.org/aos/agrovoc/c_1391, http://aims.fao.org/aos/agrovoc/c_7030, http://aims.fao.org/aos/agrovoc/c_2758, http://aims.fao.org/aos/agrovoc/c_3093, http://aims.fao.org/aos/agrovoc/c_3081, |
| Online Access: | http://agritrop.cirad.fr/389729/ |
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