Molecular marker-assisted selection of DH plants conferring genes resistant to powdery mildew in wheat (Triticum aestivum L.)
Powdery mildew , caused by Blumeria graminis f1 sp1 tritici , is one of the most destructive wheat diseases worldwide1 Using molecular markers to pyramid more effective resistance genes is one way to sustain the resistance of wheat cultivars1 The objective of this study was to identify the resistance gene pyramided in DH plants by molecular markers1 A total of 49 DH plants derived from wheat ×maize crosses and potentially containing the multiple resistance genes were tested by the STS2PCR marker of Pm4b , SCAR2PCR marker of Pm13 & PmV , and theα2amylase isoenzyme marker of Pm121 The results showed that seven plants conferred with Pm4b + Pm12 + PmV , one plant with Pm4b + Pm13 + PmV , three plants with Pm12 + Pm4b , six plants with Pm4b + PmV , two plants with Pm12 + PmV , two plants with Pm13 + Pm4b , respectively ( Table 1) 1 When using wheat ×maize crosses to produce resistance gene pyramided DH plants , it could produce more pyramided DH types through callus inducing.
| Main Authors: | , , , , , |
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| Format: | Article biblioteca |
| Language: | Chinese |
| Published: |
Institute of Crop Sciences
2005
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| Subjects: | AGRICULTURAL SCIENCES AND BIOTECHNOLOGY, Doubled Haploid, Pyramid Resistance Gene, WHEAT, POWDERY MILDEWS, HAPLOIDY, MARKER-ASSISTED SELECTION, GENETIC RESISTANCE, |
| Online Access: | http://hdl.handle.net/10883/2455 |
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