Dissecting conserved cis-regulatory modules of Glu-1 promoters which confer the highly active endosperm-specific expression via stable wheat transformation
Wheat high-molecular-weight glutenin subunits (HMW-GS) determine dough elasticity and play an essential role in processing quality. HMW-GS are encoded by Glu-1 genes and controlled primarily at transcriptional level, implemented through the interactions between cis-acting elements and trans-acting factors. However, transcriptional mechanism of Glu-1 genes remains elusive. Here we made a comprehensive analysis of cis-regulatory elements within 1-kb upstream of the Glu-1 start codon (−1000 to −1) and identified 30 conserved motifs. Based on motif distribution pattern, three conserved cis-regulatory modules (CCRMs), CCRM1 (−300 to −101), CCRM2 (−650 to −400), and CCRM3 (−950 to −750), were defined, and their functions were characterized in wheat stable transgenic lines transformed with progressive 5′ deletion promoter
| Main Authors: | , , , , , , , , , |
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| Format: | Article biblioteca |
| Language: | English |
| Published: |
Elsevier
2019
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| Subjects: | AGRICULTURAL SCIENCES AND BIOTECHNOLOGY, Conserved Cis-Regulatory Modules, Glu-1, Transcriptional Regulation, Transgenic Wheat, WHEAT, TRITICUM AESTIVUM, |
| Online Access: | https://hdl.handle.net/10883/19894 |
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