Supplementary data of the article Assessing the colonization by Daphnia magna of pesticide-disturbed habitats (chlorpyrifos, terbuthylazine and their mixtures) and the behavioral and neurotoxic effects

14 pages. -- Supplementary description of methods: 1.1. Chemical analysis of pesticides: extraction procedure and LC-MS/MS analysis; 1.2. Acetylcholinesterase (AChE) activity. -- Table S1. MRM conditions used in UPLC-MS/MS determination of pesticides selected. Two transitions (precursor ion →product ion) of each compound were analyzed. -- Table S2. Ranges of nominal and quantified concentrations in μg/L for the two pesticides used and the metabolite analyzed in the colonization assays. -- Table S3. Results of the Chi-square test to check statistically significant differences between the percentage of colonization achieved by the organisms in each chamber at 48h in the pesticide experiments and the expected value (100%). -- Table S4. Results of the within-group Kruskal-Wallis test to check statistically significant differences between chambers (source of variation) for the percentage of colonization of D. magna achieved at 48h in each pesticide experiment, together with the significant results of the pairwise post hoc test. -- Table S5. Results of the between-group Kruskal-Wallis test to check statistically significant differences between colonization experiments and chambers (sources of variation) for the percentage of colonization of D. magna at 48h, together with the significant results of the pairwise post hoc test. -- Table S6. Mean, standard error (SE), maximum and minimum values obtained for the mobility variables analyzed in D. magna after 48 h of exposure to the different study treatments. -- Table S7. Results of PERMANOVA ONE-WAY test to check statistically significant differences between study treatments for the mobility variables evaluated, together with the significant results of the pairwise post hoc test. -- Table S8. Results of Kruskal-Wallis test to check statistically significant differences between study treatments for the average vertical speed (study variable with non-normal distribution according to Shapiro-Wilk test), together with the significant results of the pairwise post hoc test and Dunn´s multiple comparisons test (specific comparisons with the control treatment). -- Table S9. One-way ANOVA test to check statistically significant differences among treatments for the remaining mobility variables evaluated, together with the significant results of the pairwise post hoc test and Dunnett´s multiple comparisons test (specific comparisons with the control treatment). -- Table S10. Mean, standard error (SE), maximum and minimum values obtained for the AChE activity in D. magna after 48 h of exposure to the different study treatments. -- Table S11. One-way ANOVA test to check statistically significant differences among treatments for the acetylcholinesterase (AChE) activity, together with the significant results of the pairwise post hoc test. -- Fig. S1. HeMHAS – Heterogeneous Multi-Habitat Assay System (version #3) used in the colonization experiments. -- Fig. S2. Detail of one of the compartments of the HeMHAS together with two gates that connect it to the adjacent chambers throughout a rotary locking system. -- Fig. S3. Picture of D. magna individually disposed in four wells of culture plates for analysis of horizontal swimming behavior. -- Fig. S4. Percentage of organisms (A) and colonization response (B) achieved in each chamber in the control test at 48h with ±SE of four replicates. -- Fig. S5. PCA analysis of the responses obtained for the mobility variables analysed in D. magna exposed to the different study treatments.

Bibliographic Details

Main Authors:	Vera-Herrera, Lucía, Araújo, Cristiano V. M., Cordero, Andrea, Blasco, Julián, Picó, Yolanda
Other Authors:	Vera-Herrera, Lucía [vehelu@uv.es]
Format:	dataset biblioteca
Language:	English
Published:	Elsevier 2022-10-15
Online Access:	http://hdl.handle.net/10261/331183
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