A fast, simple, and reliable high-yielding method for DNA extraction from different plant species

Genetic studies and pathogen detection in plants using molecular methods require the isolation of DNA from a large number of samples in a short time span. A rapid and versatile protocol for extracting high-quality DNA from different plant species is described. This method yields from 1 to 2 mg of DNA per gram of tissue. The absorbance ratios (A260/A280) obtained ranged from 1.6 to 2.0. A minimal presence of contaminating metabolites (as polymerase chain reaction [PCR] inhibitors) in samples and a considerable savings in reagents are characteristics of this protocol, as well as the low cost of the analysis per sample. The quality of the DNA was suitable for PCR amplification.

Bibliographic Details

Main Authors:	RAUL TAPIA TUSSELL, ANDRES FELIPE DE JESUS QUIJANO RAMAYO, RAFAEL ANTONIO ROJAS HERRERA, FRANCISCO ALFONSO LARQUE SAAVEDRA, DAISY DE LA CARIDAD PEREZ BRITO
Format:	info:eu-repo/semantics/article biblioteca
Language:	eng
Subjects:	info:eu-repo/classification/Autores/AGAVACEAE, info:eu-repo/classification/Autores/CARIACACEAE, info:eu-repo/classification/Autores/CUCURBITACEAE, info:eu-repo/classification/Autores/DNA ISOLATION, info:eu-repo/classification/Autores/GRAMINAEAE, info:eu-repo/classification/Autores/PALMAE, info:eu-repo/classification/cti/2, info:eu-repo/classification/cti/24,
Online Access:	http://cicy.repositorioinstitucional.mx/jspui/handle/1003/929
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