A fast, simple, and reliable high-yielding method for DNA extraction from different plant species
Genetic studies and pathogen detection in plants using molecular methods require the isolation of DNA from a large number of samples in a short time span. A rapid and versatile protocol for extracting high-quality DNA from different plant species is described. This method yields from 1 to 2 mg of DNA per gram of tissue. The absorbance ratios (A260/A280) obtained ranged from 1.6 to 2.0. A minimal presence of contaminating metabolites (as polymerase chain reaction [PCR] inhibitors) in samples and a considerable savings in reagents are characteristics of this protocol, as well as the low cost of the analysis per sample. The quality of the DNA was suitable for PCR amplification.
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| Main Authors: | , , , , |
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| Format: | info:eu-repo/semantics/article biblioteca |
| Language: | eng |
| Subjects: | info:eu-repo/classification/Autores/AGAVACEAE, info:eu-repo/classification/Autores/CARIACACEAE, info:eu-repo/classification/Autores/CUCURBITACEAE, info:eu-repo/classification/Autores/DNA ISOLATION, info:eu-repo/classification/Autores/GRAMINAEAE, info:eu-repo/classification/Autores/PALMAE, info:eu-repo/classification/cti/2, info:eu-repo/classification/cti/24, |
| Online Access: | http://cicy.repositorioinstitucional.mx/jspui/handle/1003/929 |
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