Agrobacterium-mediated transient transformation of Mexican prickly poppy (Argemone mexicana L.)

Agrobacterium-mediated transient transformation of Mexican prickly poppy (Argemone mexicana L.)

Shoot apex, leaf primordia, leaf sections and roots from Mexican prickly poppy seedlings, were inoculated with Agrobacterium tumefaciens harboring the binary vector pCAMBIA2301, which contained the β-glucuronidase (uid A) gene. Histochemical β-glucuronidase (GUS) assay in infected explants showed transient gus gene expression between 3 and 12 days after inoculation. To our knowledge, this is the first report of A. mexicana susceptibility to A. tumefaciens-mediated genetic transformation. 

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Bibliographic Details
Main Authors: GREGORIO DEL CARMEN GODOY HERNANDEZ, ELIDE AVILES BERZUNZA, MILDRED RUBI CARRILLO PECH, FELIPE AUGUSTO VAZQUEZ FLOTA
Format: info:eu-repo/semantics/article biblioteca
Language:eng
Subjects:info:eu-repo/classification/Autores/Β-GLUCURONIDASE, info:eu-repo/classification/Autores/ARGEMONE MEXICANA, info:eu-repo/classification/Autores/BENZYLISOQUINOLINE ALKALOIDS, info:eu-repo/classification/Autores/GENETIC TRANSFORMATION, info:eu-repo/classification/Autores/NEOMYCIN PHOSPHOTRANSFERASE II, info:eu-repo/classification/cti/2,
Online Access:http://cicy.repositorioinstitucional.mx/jspui/handle/1003/785
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