In vitro and in vivo anti-inflammatory properties of Mayan propolis

Introduction Propolis has been used traditionally for different human diseases and even recently as dental biomaterials because of its antibacterial, antimycotic, and anti-inflammatory properties. However, a proper correlation between in vitro and in vivo anti-inflammatory properties has not been clearly established. Methods The composition of propolis was determined by high-performance liquid chromatography-ultraviolet mass spectrometry (HPLC-UV-MS). Viability of ethanolic propolis solution was evaluated by thiazolyl blue tetrazolium bromide (MTT) assay on murine macrophages. The anti-inflammatory properties were assessed both in vitro through the enzyme-linked immunosorbent assay (ELISA) quantification of various cytokines and in vivo by induced edemas. Results Chemical analysis showed pinocembrin, pinobanksin-3-O-acetate, and pinobanksin-3-O-propionate as the main components of propolis. Macrophage viability was high (106%) when propolis was used up to 50 mu g/mL. ELISA studies showed a reduction in the expression of pro-inflammatory cytokines (IL-1 beta, IL-6, and TNF-alpha) up to 145 pg/mL, 350 pg/mL, and 210 pg/mL, respectively, while the anti-inflammatory cytokines (IL-10 and IL-4) were increased up to 833 pg/mL and 446 pg/mL. Finally, edema was reduced on paw and ear mice by 9% and 22%, respectively. Conclusion Mayan propolis has strong in vitro anti-inflammatory properties without compromising macrophage viability, resulting in a low-to-mild in vivo anti-inflammatory response.

Bibliographic Details

Main Authors:	JORGE FROYLAN XOOL TAMAYO, IVAN HUMBERTO CHAN ZAPATA, VICTOR ERMILO ARANA ARGAEZ, Fabiola Elizabeth Villa de la Torre, JULIO CESAR TORRES ROMERO, JESUS ALFREDO ARAUJO LEON, FERNANDO JAVIER AGUILAR AYALA, MARINA EDUVIGES REJON PERAZA, NELLY DEL CARMEN CASTRO LINARES, ROSSANA FARIDE VARGAS CORONADO, JUAN VALERIO CAUICH RODRIGUEZ
Format:	info:eu-repo/semantics/article biblioteca
Language:	eng
Subjects:	info:eu-repo/classification/Autores/ANTI-INFLAMMATORY, info:eu-repo/classification/Autores/CYTOKINES, info:eu-repo/classification/Autores/MACROPHAGES, info:eu-repo/classification/Autores/PROPOLIS, info:eu-repo/classification/cti/2, info:eu-repo/classification/cti/24, info:eu-repo/classification/cti/2403, info:eu-repo/classification/cti/230222,
Online Access:	http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1885
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