Flavonoid determination in the quality control of floral bioresidues from Crocus sativus L.

A high-performance liquid chromatography with photodiode array detection method (HPLC-DAD) was validated for the analysis of floral bioresidues obtained in saffron spice production by using three different solvent mixtures [water/hydrochloric acid (HCl) (100:1, v/v), water/acetonitrile/trifluoroacetic acid (47:50:3, v/v/v), and water/acetonitrile/HCl (50:50:1, v/v/v)]. Fifteen phenolic compounds were tentatively identified, kaempferol 3-O-sophoroside and delphinidin 3,5-di-O-glucoside being the main ones. The extracts showed very different phenolic profiles obtained by HPLC-DAD coupled with electrospray ionization mass spectrometry (ESI-MSn), and several experiments were carried out to explain this. The use of acetonitrile as solvent causes the chromatographic splitting of the peak of the delphinidin 3,5-di-O-glucoside into two peaks. Results obtained in this paper show that the extract prepared with water/HCl (100:1, v/v) would be the best suited for determining phenolic compounds in the quality control of the floral bioresidues from Crocus sativus L. © 2014 American Chemical Society.

Bibliographic Details

Main Authors:	Serrano-Díaz, Jéssica, Sánchez, Ana M., Martínez-Tomé, Magdalena, Winterhalter, Peter, Alonso, Gonzalo L.
Other Authors:	Junta de Comunidades de Castilla-La Mancha
Format:	artículo biblioteca
Published:	American Chemical Society 2014
Subjects:	Crocus sativus L., Kaempferol 3-O-sophoroside, Quality control, Validation, Flower waste, Delphinidin 3,5-di-O-glucoside,
Online Access:	http://hdl.handle.net/10261/99993 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100011698
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