A Prunus avium L. infusion inhibits sugar uptake and counteracts oxidative stress-induced stimulation of glucose uptake by intestinal epithelial (Caco-2) cells
This article belongs to the Special Issue The Interaction of Redox Potential Modulators with Membrane Transporters.
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| Format: | artículo biblioteca |
| Language: | English |
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Multidisciplinary Digital Publishing Institute
2024
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| Subjects: | Cherry stem, Phenolic compounds, Infusion, Antioxidant activity, Intestinal sugar uptake, |
| Online Access: | http://hdl.handle.net/10261/358808 http://dx.doi.org/10.13039/501100001871 http://dx.doi.org/10.13039/501100006111 http://dx.doi.org/10.13039/501100000780 |
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dig-cial-es-10261-3588082024-05-31T10:38:02Z A Prunus avium L. infusion inhibits sugar uptake and counteracts oxidative stress-induced stimulation of glucose uptake by intestinal epithelial (Caco-2) cells Barreto Peixoto, Juliana A. Silva, Cláudia Costa, Anabela S. G. Álvarez-Rivera, Gerardo Cifuentes, Alejandro Ibáñez, Elena Oliveira, M. Beatriz P. P. Alves, Rita C. Martel, Fátima Andrade, Nelson Fundação para a Ciência e a Tecnologia (Portugal) Ministério da Ciência, Tecnologia e Ensino Superior (Portugal) European Commission Cherry stem Phenolic compounds Infusion Antioxidant activity Intestinal sugar uptake This article belongs to the Special Issue The Interaction of Redox Potential Modulators with Membrane Transporters. Sweet cherry (Prunus avium L.) is among the most valued fruits due to its organoleptic properties and nutritional worth. Cherry stems are rich in bioactive compounds, known for their anti-inflammatory and antioxidant properties. Innumerable studies have indicated that some bioactive compounds can modulate sugar absorption in the small intestine. In this study, the phenolic profile of a cherry stem infusion was investigated, as well as its capacity to modulate intestinal glucose and fructose transport in Caco-2 cells. Long-term (24 h) exposure to cherry stem infusion (25%, v/v) significantly reduced glucose (3H-DG) and fructose (14C-FRU) apical uptake, reduced the apical-to-basolateral Papp to 3H-DG, and decreased mRNA expression levels of the sugar transporters SGLT1, GLUT2 and GLUT5. Oxidative stress (induced by tert-butyl hydroperoxide) caused an increase in 3H-DG uptake, which was abolished by the cherry stem infusion. These findings suggest that cherry stem infusion can reduce the intestinal absorption of both glucose and fructose by decreasing the gene expression of their membrane transporters. Moreover, this infusion also appears to be able to counteract the stimulatory effect of oxidative stress upon glucose intestinal uptake. Therefore, it can be a potentially useful compound for controlling hyperglycemia, especially in the presence of increased intestinal oxidative stress levels. The authors acknowledge FCT/MCTES (Fundação para a Ciência e Tecnologia and Ministério da Ciência, Tecnologia e Ensino Superior) for the national funds received through the project PTDC/SAU-NUT/2165/2021 (COBY4HEALTH—Can coffee by-products decrease the risk of metabolic syndrome? A comprehensive approach to reduce waste and valorize health benefits). C.S. is grateful to the project PTDC/SAU-NUT/2165/2021—COBY4HEALTH for her post-doc grant. J.A.B.-P. gives thanks to FCT/MCTES and ESF (European Social Fund) through NORTE 2020. N.A. and R.C.A. thanks FCT for funding through the Scientific Employment Stimulus—Individual Call (CEECIND/08492/2022 and CEECIND/01120/2017, respectively). Peer reviewed 2024-05-29T09:35:24Z 2024-05-29T09:35:24Z 2024 artículo http://purl.org/coar/resource_type/c_6501 Antioxidants 13(1): 59 (2024) http://hdl.handle.net/10261/358808 10.3390/antiox13010059 2076-3921 http://dx.doi.org/10.13039/501100001871 http://dx.doi.org/10.13039/501100006111 http://dx.doi.org/10.13039/501100000780 38247483 en Publisher's version The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.3390/antiox13010059 https://doi.org/10.3390/antiox13010059 Sí open application/pdf Multidisciplinary Digital Publishing Institute |
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Cherry stem Phenolic compounds Infusion Antioxidant activity Intestinal sugar uptake Cherry stem Phenolic compounds Infusion Antioxidant activity Intestinal sugar uptake |
| spellingShingle |
Cherry stem Phenolic compounds Infusion Antioxidant activity Intestinal sugar uptake Cherry stem Phenolic compounds Infusion Antioxidant activity Intestinal sugar uptake Barreto Peixoto, Juliana A. Silva, Cláudia Costa, Anabela S. G. Álvarez-Rivera, Gerardo Cifuentes, Alejandro Ibáñez, Elena Oliveira, M. Beatriz P. P. Alves, Rita C. Martel, Fátima Andrade, Nelson A Prunus avium L. infusion inhibits sugar uptake and counteracts oxidative stress-induced stimulation of glucose uptake by intestinal epithelial (Caco-2) cells |
| description |
This article belongs to the Special Issue The Interaction of Redox Potential Modulators with Membrane Transporters. |
| author2 |
Fundação para a Ciência e a Tecnologia (Portugal) |
| author_facet |
Fundação para a Ciência e a Tecnologia (Portugal) Barreto Peixoto, Juliana A. Silva, Cláudia Costa, Anabela S. G. Álvarez-Rivera, Gerardo Cifuentes, Alejandro Ibáñez, Elena Oliveira, M. Beatriz P. P. Alves, Rita C. Martel, Fátima Andrade, Nelson |
| format |
artículo |
| topic_facet |
Cherry stem Phenolic compounds Infusion Antioxidant activity Intestinal sugar uptake |
| author |
Barreto Peixoto, Juliana A. Silva, Cláudia Costa, Anabela S. G. Álvarez-Rivera, Gerardo Cifuentes, Alejandro Ibáñez, Elena Oliveira, M. Beatriz P. P. Alves, Rita C. Martel, Fátima Andrade, Nelson |
| author_sort |
Barreto Peixoto, Juliana A. |
| title |
A Prunus avium L. infusion inhibits sugar uptake and counteracts oxidative stress-induced stimulation of glucose uptake by intestinal epithelial (Caco-2) cells |
| title_short |
A Prunus avium L. infusion inhibits sugar uptake and counteracts oxidative stress-induced stimulation of glucose uptake by intestinal epithelial (Caco-2) cells |
| title_full |
A Prunus avium L. infusion inhibits sugar uptake and counteracts oxidative stress-induced stimulation of glucose uptake by intestinal epithelial (Caco-2) cells |
| title_fullStr |
A Prunus avium L. infusion inhibits sugar uptake and counteracts oxidative stress-induced stimulation of glucose uptake by intestinal epithelial (Caco-2) cells |
| title_full_unstemmed |
A Prunus avium L. infusion inhibits sugar uptake and counteracts oxidative stress-induced stimulation of glucose uptake by intestinal epithelial (Caco-2) cells |
| title_sort |
prunus avium l. infusion inhibits sugar uptake and counteracts oxidative stress-induced stimulation of glucose uptake by intestinal epithelial (caco-2) cells |
| publisher |
Multidisciplinary Digital Publishing Institute |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10261/358808 http://dx.doi.org/10.13039/501100001871 http://dx.doi.org/10.13039/501100006111 http://dx.doi.org/10.13039/501100000780 |
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