Carrageenan-fed and control mice microbiota DataSet
[Description of methods used for collection/generation of data] The protocol was approved by the Experimental Animal Committee of the Complutense University of Madrid and Community of Madrid (PROEX. 224.0/21). Young (n 40) (8 weeks old) ICR-CD1 female (H) and male (M) mice were housed (five per cage and separated by sex) and distributed into control (C) and experimental (CGN) groups (10 per group and sex). Mice received orally 540 mg/kg/day of κ-carrageenan resuspended in 200 μL of PBS for 15 days in the experimental groups and PBS the controls. Faecal material were collected from each mouse before (T0) and after (T2) two weeks of treatment. About 200 mg of stools were washed with 0.9% peptone water, centrifuged (10,000 ×g, 10 min) and the pellet used for genomic DNA purification. DNA was extracted using the EZNA Stool DNA Kit (Omega Bio-Tek) and quantified using the NanoDrop 1000 UV/VIS Spectrophotometer (Thermo Fisher). DNA samples were analyzed by amplicon-based metagenomic sequencing of the 16S rDNA V3-V4 region, performed by Novogen (Cambridge, UK), on an Illumina platform to generate 250 bp paired-end reads. [Dictionaries/codebooks used] The first letter of the sample code corresponds to whether the mice are female (H) or male (M). The second letter refers to whether they are carrageenan-fed mice (CGN) or control mice (C). Then, T0 or T2 is added depending on whether they are samples collected before or at the end of the treatment, respectively. J1 or J2 refers to the cage number, and the last number refers to the identification number of each mouse in each group.
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | dataset biblioteca |
| Language: | English |
| Published: |
DIGITAL.CSIC
2022
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| Subjects: | Risk assessment, Citotoxicity, Carrageenan, Gut microbiota, |
| Online Access: | http://hdl.handle.net/10261/351278 https://doi.org/10.20350/digitalCSIC/16174 |
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