Preparation of ovine and caprine β-lactoglobulin hydrolysates with ACE-inhibitory activity. Identification of active peptides from caprine β-lactoglobulin hydrolysed with thermolysin
Ovine and caprine β-lactoglobulin (β-Lg) were isolated from sweet and acid whey by precipitation with trichloroacetic acid. This method allowed a rapid purification of ovine and caprine β-Lg with high purity (higher than 92%), when starting from acid whey. These β-Lg preparations were digested with trypsin, chymotrypsin, proteinase K and thermolysin and the angiotensin converting enzyme (ACE)-inhibitory activity was determined at different hydrolysis times. Consistently, higher activity was found in the hydrolysates prepared with enzymes of microbial origin. Four novel ACE-inhibitory peptides were purified and identified from caprine β-Lg hydrolysed with thermolysin. The identified peptides were caprine β-Lg f(46–53), f(58–61), f(103–105), and f(122–125), with ACE-inhibitory activities (IC50) that ranged from 34.7 to 2470 μm. The structure of the identified active peptides in relation to previous structure–activity studies is discussed.
Main Authors: | Hernández-Ledesma, Blanca, Recio, Isidra, Ramos, Mercedes, Amigo, Lourdes |
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Other Authors: | Ministerio de Educación y Cultura (España) |
Format: | artículo biblioteca |
Published: |
Elsevier
2002
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Subjects: | Ovine and caprine whey, β-Lg hydrolysates, ACE-inhibition, Bioactive peptides, |
Online Access: | http://hdl.handle.net/10261/256549 |
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