Glutathione-stabilized silver nanoparticles: Antibacterial activity against periodontal bacteria, and cytotoxicity and inflammatory response in oral cells

Glutathione-stabilized silver nanoparticles: Antibacterial activity against periodontal bacteria, and cytotoxicity and inflammatory response in oral cells

This article belongs to the Special Issue Malignant and Potentially Malignant Disorders of the Oral Cavity: Updates from Pathogenesis to Therapy.

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Main Authors: Zorraquín-Peña, Irene, Cueva, Carolina, González de Llano, Dolores, Bartolomé, Begoña, Moreno-Arribas, M. Victoria
Other Authors: Ministerio de Economía y Competitividad (España)
Format: artículo biblioteca
Language:English
Published: Multidisciplinary Digital Publishing Institute 2020
Subjects:Silver nanoparticles, Cytokines, Oral bacteria, Cytotoxicity, Periodontal pathogens, Antimicrobial activity,
Online Access:http://hdl.handle.net/10261/228655
http://dx.doi.org/10.13039/501100011033
http://dx.doi.org/10.13039/100012818
http://dx.doi.org/10.13039/501100003329
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spelling dig-cial-es-10261-2286552021-12-27T16:29:58Z Glutathione-stabilized silver nanoparticles: Antibacterial activity against periodontal bacteria, and cytotoxicity and inflammatory response in oral cells Zorraquín-Peña, Irene Cueva, Carolina González de Llano, Dolores Bartolomé, Begoña Moreno-Arribas, M. Victoria Ministerio de Economía y Competitividad (España) Comunidad de Madrid Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) Silver nanoparticles Cytokines Oral bacteria Cytotoxicity Periodontal pathogens Antimicrobial activity This article belongs to the Special Issue Malignant and Potentially Malignant Disorders of the Oral Cavity: Updates from Pathogenesis to Therapy. Silver nanoparticles (AgNPs) have been proposed as new alternatives to limit bacterial dental plaque because of their antimicrobial activity. Novel glutathione-stabilized silver nanoparticles (GSH-AgNPs) have proven powerful antibacterial properties in food manufacturing processes. Therefore, this study aimed to evaluate the potentiality of GSH-AgNPs for the prevention/treatment of oral infectious diseases. First, the antimicrobial activity of GSH-AgNPs against three oral pathogens (Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus mutans) was evaluated. Results demonstrated the efficiency of GSH-AgNPs in inhibiting the growth of all bacteria, especially S. mutans (IC50 = 23.64 μg/mL, Ag concentration). Second, GSH-AgNPs were assayed for their cytotoxicity (i.e., cell viability) toward a human gingival fibroblast cell line (HGF-1), as an oral epithelial model. Results indicated no toxic effects of GSH-AgNPs at low concentrations (≤6.16 µg/mL, Ag concentration). Higher concentrations resulted in losing cell viability, which followed the Ag accumulation in cells. Finally, the inflammatory response in the HGF-1 cells after their exposure to GSH-AgNPs was measured as the production of immune markers (interleukins 6 and 8 (IL-6 and IL-8) and tumor necrosis factor-alpha (TNF-α)). GSH-AgNPs activates the inflammatory response in human gingival fibroblasts, increasing the production of cytokines. These findings provide new insights for the use of GSH-AgNPs in dental care and encourage further studies for their application. This research was funded by Grants AGL2015-64522-C2-R and PID2019-108851RB-C21 (Spanish Ministry of Science and Innovation) and ALIBIRD-CM 2020 P2018/BAA-4343 (Comunidad de Madrid). I.Z.-P. thanks BES-2016-077980 contract. Peer reviewed 2021-02-05T07:42:28Z 2021-02-05T07:42:28Z 2020 artículo http://purl.org/coar/resource_type/c_6501 Biomedicines 8(10): 375 (2020) http://hdl.handle.net/10261/228655 10.3390/biomedicines8100375 2227-9059 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100003329 32977686 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-64522-C2-R info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108851RB-C21 PID2019-108851RB-C21/AEI/10.13039/501100011033 P2018/BAA-4343/ALIBIRD-CM Publisher's version https://doi.org/10.3390/biomedicines8100375 Sí open Multidisciplinary Digital Publishing Institute
institution CIAL ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-cial-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del CIAL España
language English
topic Silver nanoparticles
Cytokines
Oral bacteria
Cytotoxicity
Periodontal pathogens
Antimicrobial activity
Silver nanoparticles
Cytokines
Oral bacteria
Cytotoxicity
Periodontal pathogens
Antimicrobial activity
spellingShingle Silver nanoparticles
Cytokines
Oral bacteria
Cytotoxicity
Periodontal pathogens
Antimicrobial activity
Silver nanoparticles
Cytokines
Oral bacteria
Cytotoxicity
Periodontal pathogens
Antimicrobial activity
Zorraquín-Peña, Irene
Cueva, Carolina
González de Llano, Dolores
Bartolomé, Begoña
Moreno-Arribas, M. Victoria
Glutathione-stabilized silver nanoparticles: Antibacterial activity against periodontal bacteria, and cytotoxicity and inflammatory response in oral cells
description This article belongs to the Special Issue Malignant and Potentially Malignant Disorders of the Oral Cavity: Updates from Pathogenesis to Therapy.
author2 Ministerio de Economía y Competitividad (España)
author_facet Ministerio de Economía y Competitividad (España)
Zorraquín-Peña, Irene
Cueva, Carolina
González de Llano, Dolores
Bartolomé, Begoña
Moreno-Arribas, M. Victoria
format artículo
topic_facet Silver nanoparticles
Cytokines
Oral bacteria
Cytotoxicity
Periodontal pathogens
Antimicrobial activity
author Zorraquín-Peña, Irene
Cueva, Carolina
González de Llano, Dolores
Bartolomé, Begoña
Moreno-Arribas, M. Victoria
author_sort Zorraquín-Peña, Irene
title Glutathione-stabilized silver nanoparticles: Antibacterial activity against periodontal bacteria, and cytotoxicity and inflammatory response in oral cells
title_short Glutathione-stabilized silver nanoparticles: Antibacterial activity against periodontal bacteria, and cytotoxicity and inflammatory response in oral cells
title_full Glutathione-stabilized silver nanoparticles: Antibacterial activity against periodontal bacteria, and cytotoxicity and inflammatory response in oral cells
title_fullStr Glutathione-stabilized silver nanoparticles: Antibacterial activity against periodontal bacteria, and cytotoxicity and inflammatory response in oral cells
title_full_unstemmed Glutathione-stabilized silver nanoparticles: Antibacterial activity against periodontal bacteria, and cytotoxicity and inflammatory response in oral cells
title_sort glutathione-stabilized silver nanoparticles: antibacterial activity against periodontal bacteria, and cytotoxicity and inflammatory response in oral cells
publisher Multidisciplinary Digital Publishing Institute
publishDate 2020
url http://hdl.handle.net/10261/228655
http://dx.doi.org/10.13039/501100011033
http://dx.doi.org/10.13039/100012818
http://dx.doi.org/10.13039/501100003329
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